PHAP1 Protein (His-GST)
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Quick Overview for PHAP1 Protein (His-GST) (ABIN7317458)
抗原
See all PHAP1 (ANP32A) 蛋白蛋白类型
宿主
资源
纯度
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标记
- This PHAP1 protein is labelled with His-GST.
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原理
- Recombinant Human ANP32A/PHAP1 Protein (His & GST Tag)
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序列
- Glu 2-Lys 238
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产品特性
- A DNA sequence encoding the of human ANP32A (NP_006296.1) (Glu 2-Lys 238) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
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过滤
- 0.2 μm filtered
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Biological Activity Comment
- Not validated for activity
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Acidic (Leucine-Rich) Nuclear phosphoprotein 32 Family, Member A (ANP32A) (AA 1-249) protein (GST tag)
ANP32A 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Acidic (Leucine-Rich) Nuclear phosphoprotein 32 Family, Member A (ANP32A) (AA 1-249) protein (His tag)ANP32A 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % SDS
Acidic (Leucine-Rich) Nuclear phosphoprotein 32 Family, Member A (ANP32A) (AA 1-249) protein (His-IF2DI Tag)ANP32A 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
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限制
- 仅限研究用
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状态
- Lyophilized
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缓冲液
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Lyophilized from sterile 20 mM Tris, 10 % glycerol, 1 mM DTT, 0.5 mM GSH
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization. -
储存液
- Dithiothreitol (DTT)
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注意事项
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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有效期
- 12 months
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- PHAP1 (ANP32A) (Acidic (Leucine-Rich) Nuclear phosphoprotein 32 Family, Member A (ANP32A))
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别名
- ANP32A/PHAP1
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背景
- C15orf1,HPPCn,I1PP2A,LANP,MAPM,PHAP1,PHAPI,PP32,acidic leucine-rich nuclear phosphoprotein 32 family member A, also known as acidic nuclear phosphoprotein pp32, Leucine-rich acidic nuclear protein, Mapmodulin, Potent heat-stable protein phosphatase 2A inhibitor I1PP2A, Putative HLA-DR-associated protein I, PHAPI and ANP32A, is a nucleus, cytoplasm and endoplasmic reticulum. ANP32A / LANP is expressed in all tissues tested. It is highly expressed in kidney and skeletal muscle, moderate levels of expression is in brain, placenta and pancreas. ANP32A / LANP is weakly expressed in lung. It is found in all regions of the brain examined (amygdala, caudate nucleus, corpus callosum, hippocampus and thalamus), with highest levels in amygdala. ANP32A / LANP is a component of the SET complex, which also contains SET, APEX1, HMGB2 and NME1. It directly interacts with SET. ANP32A / LANP also interacts with ATXN1/SCA1. ANP32A / LANP is implicated in a number of cellular processes, including proliferation, differentiation, caspase-dependent and caspase-independent apoptosis, suppression of transformation (tumor suppressor), inhibition of protein phosphatase 2A, regulation of mRNA trafficking and stability in association with ELAVL1, and inhibition of acetyltransferases as part of the INHAT (inhibitor of histone acetyltransferases) complex. ANP32A / LANP plays a role in E4F1-mediated transcriptional repression.
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分子量
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Calculated MW: 55.4 kDa
Observed MW: 50 kDa
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基因ID
- 8125
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NCBI登录号
- NP_006296
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UniProt
- P39687
抗原
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