Acetylcholinesterase Protein (AChE) (His tag)
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- 抗原 See all Acetylcholinesterase (AChE) 蛋白
- Acetylcholinesterase (AChE)
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 小鼠
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资源
- HEK-293 Cells
- 标记
- This Acetylcholinesterase protein is labelled with His tag.
- 原理
- Recombinant Mouse Acetylcholinesterase/ACHE Protein (His Tag)(Active)
- 序列
- Met 1-Leu 614
- 产品特性
- A DNA sequence encoding the mouse ACHE (NP_033729.1) (Met 1-Leu 614) was expressed, with a polyhistidine tag at the C-terminus.
- 纯度
- > 97 % as determined by SDS-PAGE
- 内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
- Biological Activity Comment
- Measured by its ability to cleave Acetylthiocholine.The specific activity is > 250 nmols/min/μg.
- Top Product
- Discover our top product AChE 蛋白
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Acetylcholinesterase (AChE) (AA 1-640) protein (GST tag)
AChE 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Acetylcholinesterase (AChE) (AA 515-614) protein (GST tag)AChE 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Acetylcholinesterase (AChE) (AA 32-614) protein (His tag)Crystallography grade AChE 宿主: 人 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Acetylcholinesterase (AChE) (AA 32-614) protein (His tag)Crystallography grade AChE 宿主: 小鼠 宿主: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile PBS, pH 7.4
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- 抗原
- Acetylcholinesterase (AChE)
- 别名
- Acetylcholinesterase/ACHE (AChE 产品)
- 别名
- ACEE Protein, ARACHE Protein, N-ACHE Protein, YT Protein, Ache Protein, GB14873 Protein, zgc:92550 Protein, ACE Protein, Dsim\\GD20515 Protein, GD20515 Protein, dsim_GLEANR_4292 Protein, ache Protein, mE1a Protein, mE1b Protein, mE1c Protein, mE1c-long Protein, mE1d Protein, mE1d' Protein, mE1e Protein, arache Protein, n-ache Protein, acetylcholinesterase (Cartwright blood group) Protein, acetylcholinesterase 2 Protein, acetylcholinesterase Protein, Acetylcholine esterase Protein, acetylcholinesterase (Cartwright blood group) L homeolog Protein, collagen type I alpha 2 chain Protein, ACHE Protein, AChE-2 Protein, Ache Protein, ache Protein, Dsim\Ace Protein, ache.L Protein, COL1A2 Protein, ACE-1 Protein
- 背景
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Background: Acetylcholinesterase, also known as ACHE, is an enzyme that degrades (through its hydrolytic activity) the neurotransmitter acetylcholine, producing choline and an acetate group. Acetylcholinesterase plays a crucial role in nerve impulse transmission at cholinergic synapses by rapid hydrolysis of the neurotransmitter acetylcholine (ACh). ACHE appears to be a potential therapeutic target at muscle injuries including organophosphate myopathy. It is an externally oriented membrane-bound enzyme and its main physiological role is termination of chemical transmission at cholinergic synapses and secretory organs by rapid hydrolysis of the neurotransmitter acetylcholine (ACh). ACHE plays important roles in the cholinergic system, and its dysregulation is involved in a variety of human diseases. ACHE was significantly down-regulated in the cancerous tissues of 69.2 % of hepatocellular carcinoma (HCC) patients, and the low ACHE expression in HCC was correlated with tumor aggressiveness, an elevated risk of postoperative recurrence, and a low survival rate. Both the recombinant ACHE protein and the enhanced expression of ACHE significantly inhibited HCC cell growth in vitro and tumorigenicity in vivo. ACHE as a tumor growth suppressor in regulating cell proliferation, the relevant signaling pathways, and the drug sensitivity of HCC cells. Thus, ACHE is a promising independent prognostic predictor for HCC recurrence and the survival of HCC patients. ACHE is responsible for the hydrolysis of acetylcholine in the nervous system. It is inhibited by organophosphate and carbamate pesticides. However, this enzyme is only slightly inhibited by organophosphorothionates.
Synonym: mE1a, mE1b, mE1c, mE1c-long, mE1d, mE1d', mE1e
- 分子量
- 66.2 kDa
- NCBI登录号
- NP_033729
- 途径
- Skeletal Muscle Fiber Development
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