LIF 蛋白
Quick Overview for LIF 蛋白 (ABIN7199480)
抗原
See all LIF 蛋白蛋白类型
宿主
资源
纯度
质量等级
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原理
- ActiveMax® Mouse LIF Protein, Tag Free (MALS verified)
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序列
- Ser 24 - Phe 203
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产品特性
- ActiveMax® Mouse LIF, Tag Free (LIF-M5219) is expressed from human 293 cells (HEK293). It contains AA Ser 24 - Phe 203 (Accession # P09056-1).
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内毒素水平
- Less than 1.0 EU per μg by the LAL method.
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Leukemia Inhibitory Factor (LIF) (AA 23-202) protein
LIF 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Leukemia Inhibitory Factor (LIF) (AA 24-203) (Active) proteinLIF 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS-PAGE and HPLC analyses. Active
Leukemia Inhibitory Factor (LIF) (AA 25-203) protein (Fc Tag)LIF 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Leukemia Inhibitory Factor (LIF) (Active) proteinLIF 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Leukemia Inhibitory Factor (LIF) (AA 23-202) protein (His tag)LIF 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % by SDS-PAGE gel analyses WB, ELISA
Leukemia Inhibitory Factor (LIF) (AA 24-203) protein (His tag)LIF 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Leukemia Inhibitory Factor (LIF) (AA 23-202) (Active) protein (GST tag)LIF 宿主: 人, 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % (SDS-PAGE) SDS Active
Leukemia Inhibitory Factor (LIF) (AA 1-202) protein (GST tag)LIF 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Leukemia Inhibitory Factor (LIF) proteinLIF 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by SDS-PAGE
Leukemia Inhibitory Factor (LIF) (AA 23-202) protein (His-GST)LIF 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90% by SDS-PAGE SDS
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应用备注
- This protein carries no "tag". The protein has a calculated MW of 20.0 kDa. The protein migrates as 35-45 kDa under reducing (R) condition due to glycosylation.
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限制
- 仅限研究用
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状态
- Lyophilized
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缓冲液
- PBS, pH 7.4
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储存条件
- -20 °C
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储存方法
- -20°C
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- LIF (Leukemia Inhibitory Factor (LIF))
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别名
- LIF
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背景
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Synonyms: LIF,CDF,DIA,HILDA,MLPLI,
Description: Leukemia inhibitory factor, or LIF, an interleukin 6 class cytokine, is a protein in cells that affects cell growth and development.Leukemia Inhibitory Factor has several functions such as cholinergic neuron differentiation, control of stem cell pluripotency, bone & fat metabolism, mitogenesis of factor dependent cell lines & promotion of megakaryocyte production in vivo. Removal of LIF pushes stem cells toward differentiation, but they retain their proliferative potential or pluripotency. Therefore LIF is used in mouse embryonic stem cell culture. It is necessary to maintain the stem cells in an undifferentiated state, however genetic manipulation of embryonic stem cells allows for LIF independent growth, notably overexpression of the gene Nanog. LIF is not required for culture of human embryonic stem cells. -
分子量
- 20.0 kDa
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NCBI登录号
- NP_032527
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途径
- JAK/STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Stem Cell Maintenance, Growth Factor Binding
抗原
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