This SARS-CoV-2 NSP1 protein is labelled with His tag.
应用范围
SDS-PAGE (SDS)
原理
SARS-CoV-2 (COVID-19) NSP1 Protein, His Tag
序列
AA 1-180
产品特性
SARS-CoV-2 NSP1, His Tag is expressed from E.coli cells. It contains AA Met 1 - Gly 180 (Accession # YP_009725297.1). Predicted N-terminus: Met This protein carries a polyhistidine tag at the C-terminus.
Crystallography grade
NSP1
宿主: SARS Coronavirus-2 (SARS-CoV-2)
宿主: Insect Cells
Recombinant
>95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
SDS, Crys, ELISA, WB
应用备注
Optimal working dilution should be determined by the investigator.
限制
仅限研究用
状态
Lyophilized
缓冲液
PBS, Arginine, pH 7.4
储存条件
4 °C,-20 °C,-80 °C
储存方法
For long term storage, the product should be stored at lyophilized state at -20°C or lower. This product is stable after storage at: 4-8°C for 12 months in lyophilized state, -70°C for 3 years under sterile conditions after reconstitution.
pp1ab Protein, ORF1a polyprotein;ORF1ab polyprotein Protein, ORF1ab Protein
物质类
Viral Protein
背景
The viral nonstructural protein 1 (nsP1) is the only membrane-associated protein that anchors the replication complex to the cellular membranes. NSP1 inhibits host translation by interacting with the 40S ribosomal subunit. The nsp1-40S ribosome complex further induces an endonucleolytic cleavage near the 5'UTR of host mRNAs, targeting them for degradation. Viral mRNAs are not susceptible to nsp1-mediated endonucleolytic RNA cleavage thanks to the presence of a 5'-end leader sequence and are therefore protected from degradation. By suppressing host gene expression, nsp1 facilitates efficient viral gene expression in infected cells and evasion from host immune response.