SARS-CoV-2 Spike S2 Protein (His tag)
(3 references)Quick Overview for SARS-CoV-2 Spike S2 Protein (His tag) (ABIN6952626)
抗原
See all SARS-CoV-2 Spike S2 蛋白蛋白类型
宿主
资源
应用范围
纯度
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标记
- This SARS-CoV-2 Spike S2 protein is labelled with His tag.
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原理
- SARS-CoV-2 (COVID-19) S2 protein, His Tag
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序列
- AA 686-1213
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产品特性
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SARS-CoV-2 S2 protein, His Tag is expressed from human 293 cells (HEK293). It contains AA Ser 686 - Pro 1213 (Accession # QHD43416.1). Predicted N-terminus: Ser 686
This protein carries a polyhistidine tag at the C-terminus. -
过滤
- 0.22 μm filtered
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内毒素水平
- Less than 1.0 EU per μg by the LAL method.
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SARS-CoV-2 Spike S2 (B.1.351 - beta) (Active) protein (His tag)
宿主: SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Beta 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
SARS-CoV-2 Spike S2 (ECD) protein (His tag)宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: Baculovirus infected Insect Cells Recombinant > 90 % by SDS-PAGE SDS
SARS-CoV-2 Spike S2 (B.1.1.7 - alpha) (Active) protein (His tag)宿主: SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Alpha 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
SARS-CoV-2 Spike S2 (P.1 - gamma) (Active) protein (His tag)宿主: SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Gamma 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
SARS-CoV-2 Spike S2 (Active) protein (His tag)宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
SARS-CoV-2 Spike S2 (Active) protein (His tag,AVI tag,Biotin)宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Lyophilized
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缓冲液
- PBS, pH 7.4
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注意事项
- Please avoid repeated freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
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For long term storage, the product should be stored at lyophilized state at -20°C or lower.
This product is stable after storage at:
4-8°C for 12 months in lyophilized state,
-70°C for 3 years under sterile conditions after reconstitution.
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: "An antigen microarray protocol for COVID-19 serological analysis." in: STAR protocols, Vol. 2, Issue 3, pp. 100815, (2021) (PubMed).
: "Immune responses to SARS-CoV-2 in three children of parents with symptomatic COVID-19. ..." in: Nature communications, Vol. 11, Issue 1, pp. 5703, (2020) (PubMed).
: "HDL-scavenger receptor B type 1 facilitates SARS-CoV-2 entry." in: Nature metabolism, Vol. 2, Issue 12, pp. 1391-1400, (2020) (PubMed).
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: "An antigen microarray protocol for COVID-19 serological analysis." in: STAR protocols, Vol. 2, Issue 3, pp. 100815, (2021) (PubMed).
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- SARS-CoV-2 Spike S2
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物质类
- Viral Protein
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背景
- It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
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分子量
- 60.0 kDa
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基因ID
- 43740568
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UniProt
- P0DTC2
抗原
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