Human Adenovirus type 3 (HAdV-3) 蛋白
This Recombinant Human Adenovirus type 3 protein is expressed in HEK-293 Cells.
产品编号 ABIN6941928
发货至:
中国
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Quick Overview for Human Adenovirus type 3 (HAdV-3) 蛋白 (ABIN6941928)
抗原
Human Adenovirus type 3 (HAdV-3)
蛋白类型
Recombinant
宿主
Adenovirus
资源
HEK-293 Cells
纯度
Purified virus
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原理
- Wild type Adenovirus type 3. Concentrated and purified virus particles from a double CsCl gradient purification with DNase treatment and dialysis. Safety category BSL2.
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产品特性
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Adenovirus Type 3 Particles, Wild-type
Human adenovirus type 3 particles (wild-type) produced in HEK293 cells and purified by CsCl gradient. -
纯化方法
- Human adenovirus type 3 particles (wild-type) produced in HEK293 cells and purified by CsCl gradient.
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应用备注
- 2.53x10^12 particles per mL (BCA assay)
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说明
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This product is a concentrated source of highly-purified human Adenovirus type 3 particles (wild-type) from a lysate of optimally-infected 293 cells. Following double CsCl gradient purification with DNase treatment and dialysis, this Ad3 preparation is of very high quality and minimal lot-to-lot variation.
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限制
- 仅限研究用
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状态
- Liquid
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缓冲液
- PBS, 50 mM Hepes pH 7.8, 10 % Glycerol
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储存条件
- -80 °C
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储存方法
- -80°C
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- Human Adenovirus type 3 (HAdV-3)
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别名
- Adenovirus Type 3
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物质类
- Virus
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背景
- Adenoviruses are medium-sized (80–100 nm), non-enveloped viruses. They have an icosahedral nucleocapsid containing a linear, double-stranded DNA genome of approximately 36 kb (Nermut, 1984). The viral genome is grouped into different transcriptional units, designated early (E1, E2, E3, E4), intermediate, and late. The E1 gene is essential for activation of other viral genes and for viral replication. Deletion of the E1 gene results in viruses that are replication incompetent in normal cells. However, replication-competent viral particles can be produced from E1-deleted viral vectors by providing the E1 gene in trans. The E3 gene is nonessential for either viral replication or infection (Flint, 1999).
抗原
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