EBI3 蛋白
(1 reference)
Quick Overview for EBI3 蛋白 (ABIN6700897)
抗原
See all EBI3 (IL-27b) 蛋白蛋白类型
宿主
资源
应用范围
纯度
-
-
Supplier Product No.
- 010-001-b66
-
Supplier
- Rockland
-
原理
- EBI-3 Mouse Recombinant Protein
-
纯化方法
- Recombinant protein corresponding to amino acids 2 to 207 of mature mouse EBI-3. Purity is greater than 90% as determined by reducing and non-reducing SDS-PAGE and by analytical HPLC, each against a known standard.
-
内毒素水平
- Measured by LAL is < 0.01 ng/μg or < 0.1 EU/μg protein.
-
Biological Activity Comment
- Assay data for mouse recombinant EBI-3 is based upon qualitative binding to anti-EBI-3 antibody.
-
-
Interleukin-27 subunit beta (IL-27b) (AA 1-229) protein (GST tag)
IL-27b 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Interleukin-27 subunit beta (IL-27b) proteinIL-27b 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 90,00% SDS
Interleukin-27 subunit beta (IL-27b) proteinAnimal-Free IL-27b 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 90,00% SDS
Interleukin-27 subunit beta (IL-27b) proteinIL-27b 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 90,00% SDS
Interleukin-27 subunit beta (IL-27b) proteinAnimal-Free IL-27b 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 90,00% SDS
Interleukin-27 subunit beta (IL-27b) proteinIL-27b 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant 90,00% SDS
-
-
应用备注
-
Application Note: EBI-3 protein has been tested by SDS-PAGE and is suitable as a control for polyclonal or monoclonal anti-EBI-3 in immunological assays.
Other: User Optimized
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
-
Reconstitution_Buffer: 0.02M HCl
Reconstitution_Volume: 100 μL
-
浓度
- 0.1 mg/mL
-
缓冲液
-
Buffer: 0.1 % Trifluoroacetic acid
Stabilizer: None
-
储存液
- Without preservative
-
储存条件
- -20 °C
-
储存方法
- Store vial at -20° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) . For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
-
有效期
- 6 months
-
-
-
: "Lack of p38 activation in T cells increases IL-35 and protects against obesity by promoting thermogenesis." in: EMBO reports, Vol. 25, Issue 6, pp. 2635-2661, (2024) (PubMed).
-
: "Lack of p38 activation in T cells increases IL-35 and protects against obesity by promoting thermogenesis." in: EMBO reports, Vol. 25, Issue 6, pp. 2635-2661, (2024) (PubMed).
-
- EBI3 (IL-27b) (Interleukin-27 subunit beta (IL-27b))
-
别名
- Ebi3
-
背景
-
Synonyms: Interleukin-27 subunit beta precursor [Mus musculus], mouse EBI-3 cytokine, EBI3, EBI-3, IL35, IL-35
Background: Epstein-Barr Virus Induced Gene-3 (EBI-3), is a secreted glycoprotein belonging to the hematopoietin receptor family and related to the p40 subunit of IL-12. It was identified by its induced expression in B-lymphocytes in response to Epstein-Barr virus infection. EBI-3 forms heterodimers with p28 to form IL-27 and with p35 to form IL-35. Both IL-27 and IL-35 have anti-inflammatory and regulatory activity. Recombinant Mouse EBI is a non-glycosylated polypeptide chain consisting of 207 amino acids with a molecular weight of 22,900 Da.
-
基因ID
- 50498
-
UniProt
- O35228
抗原
-
