Vimentin Protein (VIM) (His tag)
Quick Overview for Vimentin Protein (VIM) (His tag) (ABIN6700879)
抗原
See all Vimentin (VIM) 蛋白蛋白类型
宿主
资源
应用范围
纯度
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标记
- This Vimentin protein is labelled with His tag.
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Supplier Product No.
- 009-001-e54s
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Supplier
- Rockland
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原理
- Vimentin recombinant protein-HIS Epitope
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纯化方法
- Recombinant full-length human Vimentin was expressed by E. coli cells using an N-terminal his epitope. The purity was determined to be >95% by densitometry.
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Vimentin (VIM) (AA 1-466) protein (GST tag)
VIM 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Vimentin (VIM) (AA 2-201) protein (His tag)VIM 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Vimentin (VIM) (AA 41-140) protein (GST tag)VIM 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Vimentin (VIM) (AA 2-466) protein (His tag)VIM 宿主: 人 宿主: 酵母菌 Recombinant > 90 % ELISA
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应用备注
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Application Note: Vimentin Protein is stored in 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25 % glycerol. Vimentin Protein is suitable for use in Western Blot. Expect a band approximately ~60 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.
Western Blot Dilution: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.2 μg/μL
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缓冲液
- Vimentin Protein is stored in 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25 % glycerol.
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储存条件
- -80 °C
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储存方法
- Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
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有效期
- 12 months
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- Vimentin (VIM)
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别名
- VIM
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背景
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Synonyms: VIM, FLJ36605, Vimentin
Background: Vimentin is a member of intermediate filament family of proteins and is an important structural feature of eukaryotic cells (1). Along with microtubules and actin microfilaments, Vimentin make up the cytoskeleton component of cells. Studies have shown that Vimentin is attached to the nucleus, endoplasmic reticulum and mitochondria, either laterally or terminally. Vimentin plays a significant role in supporting and anchoring the position of the organelles in the cytosol (2). Thus, Vimentin plays a key role in maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. Vimentin Protein is ideal for investigators involved in Signaling Proteins, Microtubule/Actin Associated Proteins, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, Cell Cycle, Cellular Stress, Inflammation, Invasion/Metastasis, and Neurobiology research.
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NCBI登录号
- NM_003380
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途径
- Caspase Cascade in Apoptosis
抗原
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