CrkL Protein (His tag)
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北京 101111
Quick Overview for CrkL Protein (His tag) (ABIN6700844)
抗原
See all CrkL (CRKL) 蛋白蛋白类型
宿主
资源
应用范围
纯度
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标记
- This CrkL protein is labelled with His tag.
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Supplier Product No.
- 009-001-r74s
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Supplier
- Rockland
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原理
- CRKL recombinant protein-HIS Epitope
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纯化方法
- Recombinant full length human CRKL was expressed by baculovirus in Sf9 insect cells using an N-Terminal his epitope. The purity was determined to be >80% by densitometry.
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!探索我们的预定义定制蛋白和定制蛋白服务！Product表达系统ConjugateOrigin价格从表达系统 HEK-293 CellsConjugate His tagOrigin Human价格从 127,054.94 ¥表达系统 Cell-free protein synthesis (CFPS)Conjugate Strep TagOrigin Human价格从 145,985.55 ¥您的项目需要进一步定制吗？联系我们，了解我们的定制蛋白解决方案
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V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL) (AA 1-303) protein (His tag)
custom-made CRKL 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL) (AA 1-303) protein (His tag)custom-made CRKL 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL) (AA 1-303) protein (His-IF2DI Tag)CRKL 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. WB, ELISA
V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL) (AA 1-303) protein (GST tag)CRKL 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL) (AA 1-303) protein (His tag)CRKL 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS
V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL) protein (Myc-DYKDDDDK Tag)CRKL 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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应用备注
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Application Note: CRKL Protein is stored in 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25 % glycerol. CRKL Protein is suitable for use in Western Blot. Expect a band approximately ~ 39 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.
Western Blot Dilution: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.2 μg/μL
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缓冲液
- CRKL Protein is stored in 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25 % glycerol.
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储存条件
- -80 °C
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储存方法
- Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
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有效期
- 12 months
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- CrkL (CRKL) (V-Crk Sarcoma Virus CT10 Oncogene Homolog (Avian)-Like (CRKL))
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别名
- CRKL
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背景
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Synonyms: crk-like protein, Crk-like protein
Background: CRKL or v-crk sarcoma virus CT10 oncogene homolog (avian)-like is a protein kinase containing SH2 and SH3 (src homology) domains that activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion (1). It is a substrate of the BCR-ABL tyrosine kinase which plays a role in fibroblast transformation by BCR-ABL, and may be oncogenic (2). CRKL is also required for normal cellular responses to Fgf8, including survival and migration, Erk activation, and target gene expression. CRKL Protein is ideal for investigators involved in Signaling Proteins, Cellular Proteins, Apoptosis/Autophagy, Cancer, Cell Cycle, Receptor Tyrosine Kinases, and WNT Signaling research.
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NCBI登录号
- NM_005207
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途径
- Interferon-gamma Pathway, Neurotrophin Signaling Pathway, Signaling of Hepatocyte Growth Factor Receptor
抗原
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