YWHAE 蛋白
Quick Overview for YWHAE 蛋白 (ABIN6700776)
抗原
See all YWHAE 蛋白蛋白类型
宿主
资源
应用范围
纯度
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Supplier Product No.
- 009-001-r26s
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Supplier
- Rockland
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原理
- 14-3-3 epsilon recombinant protein
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纯化方法
- Recombinant full-length human tag-free 14-3-3ε was expressed in E. coli cells. The purity was determined to be >90% by densitometry.
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!探索我们的预定义定制蛋白和定制蛋白服务！Product表达系统ConjugateOrigin价格从表达系统 HEK-293 CellsConjugate His tagOrigin Human价格从 115,504.49 ¥表达系统 Cell-free protein synthesis (CFPS)Conjugate Strep TagOrigin Human价格从 172,528.32 ¥您的项目需要进一步定制吗？联系我们，了解我们的定制蛋白解决方案
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14-3-3 epsilon (YWHAE) (AA 1-255) protein (His tag)
custom-made YWHAE 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
14-3-3 epsilon (YWHAE) (AA 1-255) protein (His tag)custom-made YWHAE 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
14-3-3 epsilon (YWHAE) (AA 1-255) protein (His tag)YWHAE 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
14-3-3 epsilon (YWHAE) (AA 1-255) protein (GST tag)YWHAE 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
14-3-3 epsilon (YWHAE) protein (GST tag)YWHAE 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % as determined by SDS-PAGE
14-3-3 epsilon (YWHAE) (AA 1-255) proteinYWHAE 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % pure SDS
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应用备注
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Application Note: 14-3-3ε Protein is stored in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol. 14-3-3ε Protein is suitable for use in Western Blot. Expect a band approximately ~30 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.
Western Blot Dilution: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.2 μg/μL
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缓冲液
- 14-3-3ε Protein is stored in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.
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储存条件
- -80 °C
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储存方法
- Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
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有效期
- 12 months
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- YWHAE (14-3-3 epsilon (YWHAE))
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别名
- YWHAE
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背景
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Synonyms: 14-3-3 epsilon, YWHAE, MDS, MDCR, KCIP-1, 14-3-3E, FLJ45465
Background: 14-3-3ε also known as tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein is a member of the 14-3-3 family. 14-3-3ε has been reported to be involved in heat shock transcription factor 1 (HSF1) and extracellular signal regulated protein kinase (ERK) pathways (1). 14-3-3ε interaction with the deubiquitinating enzyme UBPY, also known as USP8, regulates cargo sorting and membrane traffic at early endosomes. Association of 14-3-3ε, HSF1 with ERK during heat shock may modulate the amplitude of the response and control the termination of HSP expression on resumption of growth conditions (2). 14-3-3ε protein is ideal for investigators involved in Cell Stress & Chaperone Proteins, Cell Signaling, Cancer research, Cell Cycle, Cellular Stress, Neurobiology, and WNT Signaling research.
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NCBI登录号
- NM_006761
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途径
- Neurotrophin Signaling Pathway, Myometrial Relaxation and Contraction, M Phase
抗原
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