YWHAB 蛋白
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- 抗原 See all YWHAB 蛋白
- YWHAB (14-3-3 alpha + beta (YWHAB))
- 蛋白类型
- Recombinant
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 应用范围
- Western Blotting (WB)
- Supplier Product No.
- 009-001-r24s
- Supplier
- Rockland
- 原理
- 14-3-3 alpha/beta recombinant protein
- 产品特性
- 14-3-3 alpha/beta, YWHAB, HS1, GW128, KCIP-1, Protein 1054, Protein kinase C inhibitor protein 1, KCIP-1
- 纯化方法
- Recombinant full-length human 14-3-3α/β was expressed in E.coli cells. The purity was determined to be >90% by densitometry.
- Top Product
- Discover our top product YWHAB 蛋白
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14-3-3 alpha + beta (YWHAB) (AA 1-246) protein (His tag)
custom-made YWHAB 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
14-3-3 alpha + beta (YWHAB) (AA 1-246) protein (His tag)custom-made YWHAB 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
14-3-3 alpha + beta (YWHAB) (AA 1-246) protein (His tag)YWHAB 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
14-3-3 alpha + beta (YWHAB) (AA 1-246) protein (GST tag)YWHAB 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
14-3-3 alpha + beta (YWHAB) (AA 1-246) proteinYWHAB 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % pure SDS
14-3-3 alpha + beta (YWHAB) (AA 1-246), (full length) protein (GST tag)YWHAB 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 95 % ELISA
14-3-3 alpha + beta (YWHAB) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)YWHAB 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
14-3-3 alpha + beta (YWHAB) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)YWHAB 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
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Application Note: 14-3-3 alpha/beta Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol. 14-3-3 alpha/beta Protein is suitable for use in Western Blot. Expect a band approximately ~29 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.
Western Blot Dilution: User Optimized
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.2 μg/μL
- 储存条件
- -80 °C
- 储存方法
- Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
- 有效期
- 12 months
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- 抗原
- YWHAB (14-3-3 alpha + beta (YWHAB))
- 别名
- 14-3-3 alpha/beta (YWHAB 产品)
- 背景
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Synonyms: 14-3-3 alpha/beta, YWHAB, HS1, GW128, KCIP-1, Protein 1054, Protein kinase C inhibitor protein 1, KCIP-1
Background: 14-3-3α/β is a member of the highly conserved 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins (1). 14-3-3α/β protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. 14-3-3-β interacts with the TSC1-TSC2 dimer (2). The interaction required phosphorylation of TSC2 at Ser1210. Binding of 14-3-3-β to TSC2 did not alter the interaction between TSC1 and TSC2, but it reduced the ability of the complex to inhibit phosphorylation of ribosomal protein S6 kinase impairing the ability of the complex to inhibit cell growth. 14-3-3 alpha/beta Protein is ideal for investigators involved in Cell Stress & Chaperone Proteins, Cell Signaling, Cancer research, Cell Cycle, Cellular Stress, Neurobiology, and WNT Signaling research.
- NCBI登录号
- NM_003404
- 途径
- Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Myometrial Relaxation and Contraction, Maintenance of Protein Location
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