SIRT5 Protein (GST tag)
Quick Overview for SIRT5 Protein (GST tag) (ABIN6700535)
抗原
See all SIRT5 蛋白蛋白类型
宿主
资源
应用范围
纯度
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标记
- This SIRT5 protein is labelled with GST tag.
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Supplier Product No.
- 009-001-t35s
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Supplier
- Rockland
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原理
- SIRT5 recombinant protein-GST fusion protein
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纯化方法
- Full-length recombinant human SIRT5 was expressed in E. coli cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >95% by densitometry.
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Sirtuin 5 (SIRT5) (AA 34-310) protein (His tag)
SIRT5 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Sirtuin 5 (SIRT5) (AA 1-310) protein (GST tag)SIRT5 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Sirtuin 5 (SIRT5) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)SIRT5 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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应用备注
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Application Note: SIRT5 Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol. SIRT5 Protein is suitable for use in Western Blot. Expect a band approximately ~60 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.
Western Blot Dilution: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.2 μg/μL
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缓冲液
- SIRT5 Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.
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储存条件
- -80 °C
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储存方法
- Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
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有效期
- 12 months
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- SIRT5 (Sirtuin 5 (SIRT5))
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别名
- SIRT5
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背景
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Synonyms: SIR2L5, Sirtuin 5, NAD-dependent protein deacylase sirtuin-5, mitochondrial, Regulatory protein SIR2 homolog 5, SIR2-like protein 5, SIRT5, SIR2L5
Background: SIRT5 is a member of the sirtuin family of proteins which are homologs to the yeast Sir2 protein. Sirtuin family contain a sirtuin core domain and are grouped into four classes with SIRT5 being a member of class III. SIRT5 consists of eight exons and is found in two isoforms which encode a 310 aa and a 299 aa protein, respectively. Human SIRT5 is most predominantly expressed in heart muscle cells and in lymphoblasts. Fluorescence in situ hybridization analysis localized the human SIRT5 gene to chromosome 6p23. SIRT5 can deacetylate cytochrome c, a protein of the mitochondrial intermembrane space with a central function in oxidative metabolism as well as apoptosis initiation (1). SIRT5 Protein is ideal for investigators involved in Signaling Proteins, Deacetylase/Demethylase Proteins, Cancer, Cell Cycle, and Inflammation research.
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NCBI登录号
- NM_012241
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途径
- SARS-CoV-2 Protein Interactome
抗原
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