SIRT4 Protein (GST tag)
Quick Overview for SIRT4 Protein (GST tag) (ABIN6700533)
抗原
See all SIRT4 蛋白蛋白类型
宿主
资源
应用范围
纯度
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标记
- This SIRT4 protein is labelled with GST tag.
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Supplier Product No.
- 009-001-t34s
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Supplier
- Rockland
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原理
- SIRT4 recombinant protein-GST fusion protein
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纯化方法
- Full-length recombinant human SIRT4 was expressed in E. coli cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >70% by densitometry.
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Sirtuin 4 (SIRT4) (AA 1-314) protein (His-IF2DI Tag)
SIRT4 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Sirtuin 4 (SIRT4) (AA 1-314) protein (GST tag)SIRT4 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Sirtuin 4 (SIRT4) proteinSIRT4 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % WB, SDS, ELISA, IP
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应用备注
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Application Note: SIRT4 Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol. SIRT4 Protein is suitable for use in Western Blot. Expect a band approximately ~62 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.
Western Blot Dilution: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.2 μg/μL
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缓冲液
- SIRT4 Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.
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储存条件
- -80 °C
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储存方法
- Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
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有效期
- 12 months
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- SIRT4 (Sirtuin 4 (SIRT4))
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别名
- SIRT4
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背景
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Synonyms: IR2L4, MGC57437, MGC130046, MGC130047, sirtuin 4, NAD-dependent protein deacetylase sirtuin-4, NAD-dependent ADP-ribosyltransferase sirtuin-4, Regulatory protein SIR2 homolog 4, SIR2-like protein 4, SIRT4, SIR2L4
Background: SIRT4 is a member of the sirtuin family of proteins which are homologs to the yeast Sir2 protein. Sirtuin family contain a sirtuin core domain and are grouped into four classes with SIRT4 being a member of class IV. SIRT4 lacks deacetylase activity but has ADP-ribosyltransferase activity (1). Immunoprecipitation analysis shows that SIRT4 interacts with the mitochondrial enzyme glutamate dehydrogenase (GDH), and functional analysis show that SIRT4 ADP-ribosylates and inhibits GDH (2). Downregulation of SIRT4 by RNA interference activates GDH, thereby upregulating insulin secretion in response to glucose and amino acids. SIRT4 Protein is ideal for investigators involved in Signaling Proteins, Deacetylase/Demethylase Proteins, Cancer, Cell Cycle, and Inflammation research.
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NCBI登录号
- NM_012240
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途径
- Negative Regulation of Hormone Secretion
抗原
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