SIRT2 Protein (GST tag)
Quick Overview for SIRT2 Protein (GST tag) (ABIN6700529)
抗原
See all SIRT2 蛋白蛋白类型
宿主
资源
应用范围
纯度
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标记
- This SIRT2 protein is labelled with GST tag.
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Supplier Product No.
- 009-001-t32s
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Supplier
- Rockland
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原理
- SIRT2 recombinant protein-GST fusion protein
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纯化方法
- Full-length recombinant human SIRT2 was expressed in E. coli cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >85% by densitometry.
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Sirtuin 2 (SIRT2) (AA 2-389) protein (His tag)
SIRT2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Sirtuin 2 (SIRT2) (AA 1-352) protein (GST tag)SIRT2 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Sirtuin 2 (SIRT2) (AA 1-352) protein (His tag)SIRT2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90% by SDS-PAGE SDS
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应用备注
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Application Note: SIRT2 Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol. SIRT2 Protein is suitable for use in Western Blot. Expect a band approximately ~64 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.
Western Blot Dilution: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.2 μg/μL
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缓冲液
- SIRT2 Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.
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储存条件
- -80 °C
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储存方法
- Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
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有效期
- 12 months
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- SIRT2 (Sirtuin 2 (SIRT2))
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别名
- SIRT2
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背景
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Synonyms: Regulatory protein SIR2 homolog 2, SIR2-like protein 2, SIRT2, SIR2L, SIR2L2, Sirtuin 2
Background: SIRT2 is a member of the sirtuin family of proteins which are homologs to the yeast Sir2 protein. Sirtuin family contain a sirtuin core domain and are grouped into four classes with SIRT2 being a member of class I. Inhibition of SIRT2 can lead to neuroprotection in cellular and invertebrate models of Huntington's disease (1). Huntington's disease is characterized by increased sterols synthesis in neuronal cells and this process is reversed by SIRT2 inhibition. SIRT2 can deacetylate lys40 of alpha-tubulin both in vitro and in vivo (2). Knockdown of SIRT2 via small interfering RNA results in tubulin hyperacetylation. SIRT2 Protein is ideal for investigators involved in Signaling Proteins, Deacetylase/Demethylase Proteins, Cancer, Cell Cycle, and Inflammation research.
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NCBI登录号
- NM_030593
抗原
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