PRMT5 Protein (GST tag)
Quick Overview for PRMT5 Protein (GST tag) (ABIN6700421)
抗原
See all PRMT5 蛋白蛋白类型
宿主
资源
应用范围
纯度
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标记
- This PRMT5 protein is labelled with GST tag.
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Supplier Product No.
- 009-001-t05s
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Supplier
- Rockland
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原理
- PRMT5 recombinant protein-GST fusion protein
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纯化方法
- Recombinant full-length human PRMT5 was expressed by baculovirus in Sf9 insect cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >95% by densitometry.
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Protein Arginine Methyltransferase 5 (PRMT5) (AA 1-637) protein (His tag)
custom-made PRMT5 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Protein Arginine Methyltransferase 5 (PRMT5) (AA 1-637) protein (GST tag)PRMT5 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Protein Arginine Methyltransferase 5 (PRMT5) (AA 531-637) protein (GST tag)PRMT5 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Protein Arginine Methyltransferase 5 (PRMT5) protein (DYKDDDDK Tag)PRMT5 宿主: 人 宿主: Baculovirus Recombinant EAA, ScA
Protein Arginine Methyltransferase 5 (PRMT5) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)PRMT5 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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应用备注
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Application Note: PRMT5 Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol. PRMT5 Protein is suitable for use in Western Blot and Kinase Assay. Expect a band approximately ~96 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.
Western Blot Dilution: User Optimized
Other: Kinase Assay-User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.1 μg/μL
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缓冲液
- PRMT5 Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.
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储存条件
- -80 °C
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储存方法
- Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
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有效期
- 12 months
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- PRMT5 (Protein Arginine Methyltransferase 5 (PRMT5))
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别名
- PRMT5
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背景
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Synonyms: HRMT1L5, IBP72, JBP1, SKB1, SKB1Hs, Protein arginine N-methyltransferase 5, 72 kDa ICln-binding protein, Histone-arginine N-methyltransferase PRMT5, Jak-binding protein 1, Shk1 kinase-binding protein 1 homolog, SKB1 homolog, SKB1Hs, Protein arginine N-methyltransferase 5, N-terminally processed
Background: PRMT5 is a protein arginine methyltransferase and part of a 20S methyltransferase complex that modifies specific arginines to dimethylarginines in several spliceosomal Sm proteins (1). This modification targets Sm proteins to the survival of motor neurons (SMN) complex for assembly into small nuclear ribonucleoprotein core particles. MEP50 associates with PRMT5 in the 20S arginine methyltransferase complex in HeLa cells (2). Antibodies directed against MEP50 can reduce the arginine methyltransferase activity of the immunopurified complex toward Sm substrates. PRMT5-mediated transcriptional repression of reporter gene is dependent on MEP50. PRMT5 Protein is ideal for investigators involved in Signaling Proteins, Acetyl/Methyltransferase Proteins, Apoptosis/Autophagy, Cancer, Cell Cycle, and Neurobiology research.
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NCBI登录号
- NM_006109
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途径
- Chromatin Binding, Regulation of Muscle Cell Differentiation, Ribonucleoprotein Complex Subunit Organization, Skeletal Muscle Fiber Development
抗原
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