Quick Overview for PDGF 蛋白 (ABIN6700388)
抗原
蛋白类型
宿主
资源
应用范围
纯度
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Supplier Product No.
- 009-001-w10-0010
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Supplier
- Rockland
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原理
- Human Platelet Derived Growth Factor-AB Recombinant Protein
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纯化方法
- platelet Derived Growth Factor-AB purity was determined to be greater than 95% as determined by analysis by UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-pAGE.
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内毒素水平
- Measured by LAL is typically ≤ 1 EU/μg protein.
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Biological Activity Comment
- The activity is determined by the dose-dependent proliferation of mouse 3T3 indicator cells and is typically less than 1 ng/mL.
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PDGF protein
宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 95,00% SDS
PDGF proteinAnimal-Free 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 95,00% SDS
PDGF proteinAnimal-Free 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 95,00% SDS
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应用备注
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Application Note: Platelet Derived Growth Factor-AB Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Platelet Derived Growth Factor-AB in immunological assays.
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 10 μL (10-100 μL)
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浓度
- 0.1 mg/mL
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缓冲液
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Buffer: 0.1 % Trifluoroacetic acid
Stabilizer: None
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储存液
- Without preservative
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储存条件
- -20 °C
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储存方法
- Store vial at -20° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
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有效期
- 6 months
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- PDGF
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别名
- PDGFA/PDGFB
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背景
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Synonyms: PDGF-1, Platelet-derived growth factor A chain, Platelet-derived growth factor alpha polypeptide, PDGF-2, Platelet-derived growth factor B chain, Platelet-derived growth factor beta polypeptide, proto-oncogene c-Sis
Background: Platelet-Derived Growth Factor (PDGF) is a mitogenic peptide growth hormone carried in the alpha-granules of platelets and is released when platelets adhere to traumatized tissues. Connective tissue cells near the traumatized region respond by initiating the process of replication. The synthesis of PDGF can be induced by IL-1, IL-6, TNF-α, TGF-β and EGF. Recombinant human PDGF-AB is a non-glycosylated, disulfide-linked heterodimer, containing one 14.3 kDa alpha-chain and one 12.1 kDa beta-chain, with total molecular weight of 26.4 kDa.
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UniProt
- P04085
抗原
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