PAKRK2 protein (GST tag)
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北京 101111
Quick Overview for PAKRK2 protein (GST tag) (ABIN6700344)
抗原
蛋白类型
宿主
资源
应用范围
纯度
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标记
- GST tag
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Supplier Product No.
- 009-001-s80s
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Supplier
- Rockland
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原理
- Parkin recombinant protein-GST fusion protein
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纯化方法
- Recombinant full-length human Parkin was expressed by baculovirus in Sf9 insect cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >75% by densitometry.
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应用备注
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Application Note: Parkin Protein is stored in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol. Parkin Protein is suitable for use in Western Blot. Expect a band approximately ~68 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.
Western Blot Dilution: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.1 μg/μL
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缓冲液
- Parkin Protein is stored in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.
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储存条件
- -80 °C
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储存方法
- Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
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有效期
- 12 months
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- PAKRK2
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背景
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Synonyms: PARK2, AR-JP, PDJ, PRKN, LPRS2,E3 ubiquitin-protein ligase parkin
Background: Parkin or parkinson protein 2 is a RING domain-containing E3 ubiquitin protein ligase involved in proteasome-dependent degradation of proteins. Mutations in Parkin can lead to Parkinson's disease. Parkin is important for mitochondrial quality control by lysosome-dependent degradation of damaged mitochondria through autophagy, or mitophagy (1). Parkin plays an important role in directly supporting mitochondrial function and protecting mitochondrial genomic integrity from oxidative stress (2). Parkin also has essential role in regulating normal respiratory function in mitochondria and in the protection of cells from oxidative stress. PARK7 Protein is ideal for investigators involved in Signaling Proteins, Ubiquitin Proteins, Apoptosis/Autophagy, Cancer, Cell Cycle, and Neurobiology research.
抗原
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