MyI9 protein (GST tag)
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北京 101111
Quick Overview for MyI9 protein (GST tag) (ABIN6700272)
抗原
蛋白类型
宿主
资源
应用范围
纯度
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标记
- GST tag
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Supplier Product No.
- 010-001-s38
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Supplier
- Rockland
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原理
- LC20 recombinant protein-GST fusion protein
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纯化方法
- Recombinant full-length mouse LC20 was expressed in E. coli cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >95% by densitometry.
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应用备注
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Application Note: LC20 Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol. LC20 Protein is suitable for use in Western Blot and Kinase Assay. Expect a band approximately ~45 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.
Western Blot Dilution: User Optimized
Other: Kinase Assay-User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.2 μg/μL
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缓冲液
- LC20 Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.
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储存条件
- -80 °C
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储存方法
- Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
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有效期
- 12 months
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- MyI9
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背景
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Synonyms: MYL9, MGC3505, MLC2, MRLC1, MYRL2, Myosin regulatory light polypeptide 9, Myosin regulatory light chain 2, smooth muscle isoform, Myosin regulatory light chain 9
Background: LC20 is the myosin light chain that may regulate muscle contraction by modulating the ATPase activity of myosin heads (1). LC20 protein binds calcium and is activated by myosin light chain kinase. Two transcript variants encoding different isoforms have been found for LC20 and the deduced 172-amino acid protein is highly conserved, with only 3 differences between the human and chicken proteins. Light chain phosphorylation causes the folded monomeric form of myosin to extend and assemble into filaments. This observation established the involvement of the LC20 in conformational transitions of smooth muscle myosin (2). LC20 Protein is ideal for investigators involved in Signaling Reagents, Protein Substrates, and Cardiovascular Disease research.
抗原
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