Moesin Protein (MSN) (GST tag)
Quick Overview for Moesin Protein (MSN) (GST tag) (ABIN6700268)
抗原
See all Moesin (MSN) 蛋白蛋白类型
宿主
资源
应用范围
纯度
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标记
- This Moesin protein is labelled with GST tag.
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Supplier Product No.
- 009-001-s50
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Supplier
- Rockland
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原理
- Moesin recombinant protein-GST fusion protein
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纯化方法
- Recombinant human Moesin (410-end) was expressed in E. coli cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >95% by densitometry.
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Moesin (MSN) (AA 1-577) protein (GST tag)
MSN 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Moesin (MSN) (AA 410-577) protein (His-IF2DI Tag)MSN 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Moesin (MSN) (AA 1-577) protein (His tag)MSN 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Moesin (MSN) protein (Myc-DYKDDDDK Tag)MSN 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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应用备注
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Application Note: Moesin Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol. Moesin Protein is suitable for use in Western Blot and Kinase Assay. Expect a band approximately ~50 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.
Western Blot Dilution: User Optimized
Other: Kinase Assay-User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.2 μg/μL
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缓冲液
- Moesin Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.
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储存条件
- -80 °C
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储存方法
- Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
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有效期
- 12 months
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- Moesin (MSN)
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别名
- MSN
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背景
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Synonyms: MSN, Moesin Membrane-organizing extension spike protein
Background: Moesin (or membrane-organizing extension spike protein) belongs to ERM family that modulates epithelial integrity by regulating cell-signalling events that affect actin organization and polarity (1). The effects of Moesin on epithelial cells appear to result from inhibition of Rho signaling. ERM proteins serve a structural role in linkage of the cytoskeleton to the plasma membrane and the rescue of cells lacking Moesin by modulation of Rho signaling indicates that inhibition of Rho activity may be a more critical function of Moesin. The negative feedback loop produced by Rho's activation of ERM may be an important mechanism that prevents the excessive migratory and invasive properties characteristic of metastatic cancer cells (2). Moesin Protein is ideal for investigators involved in Signaling Reagents, Protein Substrates, Inflammation, and PKA/PKC Pathway research.
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NCBI登录号
- NM_002444
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途径
- Asymmetric Protein Localization
抗原
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