Quick Overview for MIF 蛋白 (ABIN6700252)
抗原
See all MIF 蛋白蛋白类型
宿主
资源
应用范围
纯度
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Supplier Product No.
- 009-001-v79-0005
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Supplier
- Rockland
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原理
- Human Migration Inhibitory Factor Recombinant Protein
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纯化方法
- Migration Inhibitory Factor purity was determined to be greater than 97% as determined by HpLC, analysis by UV-Spectroscopy at 280nm, and by reducing and non-reducing SDS-pAGE.
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内毒素水平
- Measured by LAL is typically ≤ 1 EU/μg protein.
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Biological Activity Comment
- The activity was measured in an agarose microdroplet assay using human U937 and production of IL-8 by human PBMCs.
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Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) (Active) protein
MIF 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) (AA 2-115) protein (His tag)MIF 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) (AA 2-115) protein (Fc Tag)MIF 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) (Active) proteinMIF 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) (AA 1-115) protein (His tag)MIF 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) (AA 1-115) protein (His tag)MIF 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) (AA 2-115) protein (His-IF2DI Tag)MIF 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) (AA 1-115) protein (GST tag)MIF 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) protein (His tag)MIF 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by SDS-PAGE
Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF) (AA 1-115) protein (GST tag)MIF 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % by SDS - PAGE SDS
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应用备注
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Application Note: Migration Inhibitory Factor Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Migration Inhibitory Factor in immunological assays.
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 5 μL (5-50 μL)
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缓冲液
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Buffer: 0.01 M Sodium Phosphate, pH 7.5
Stabilizer: None
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储存液
- Without preservative
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
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有效期
- 6 months
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- MIF (Macrophage Migration Inhibitory Factor (Glycosylation-Inhibiting Factor) (MIF))
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别名
- MIF
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背景
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Synonyms: Glycosylation-inhibiting factor (GIF), L-dopachrome isomerase, L-dopachrome tautomerase, Phenylpyruvate tautomerase
Background: Migration Inhibitory Factor (MIF) is a pro-inflammatory cytokine that acts on fibroblasts by inducing IL-1, IL-8 and MMP expression. MIF stimulates NO production and TNF-α release following IFNγ-activation of macrophages. Recombinant human MIF is a non-glycosylated protein, containing 115 amino acids, with a molecular weight of 12.5 kDa.
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UniProt
- P07141
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途径
- Regulation of Systemic Arterial Blood Pressure by Hormones, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Smooth Muscle Cell Migration, Negative Regulation of intrinsic apoptotic Signaling
抗原
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