Quick Overview for LIF 蛋白 (ABIN6700220)
抗原
See all LIF 蛋白蛋白类型
宿主
资源
应用范围
纯度
质量等级
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Supplier Product No.
- 009-f01-v72-0005
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Supplier
- Rockland
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原理
- Human Leukemia Inhibitory Factor Recombinant Protein (Animal Free)
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纯化方法
- Human Leukemia Inhibitory Factor is produced with no animal-derived raw products, animal free equipment and animal free protocols. Purity was determined to be greater than 98% as determined by reducing and non-reducing SDS-PAGE.
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内毒素水平
- Measured by LAL is typically ≤ 1 EU/μg protein.
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Biological Activity Comment
- The activity is determined by its ability to induce proliferation of TF-1 cells and is typically less than 0.07 ng/mL.
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Leukemia Inhibitory Factor (LIF) (AA 23-202) protein
LIF 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Leukemia Inhibitory Factor (LIF) (AA 24-203) (Active) proteinLIF 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS-PAGE and HPLC analyses. Active
Leukemia Inhibitory Factor (LIF) (AA 25-203) protein (Fc Tag)LIF 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Leukemia Inhibitory Factor (LIF) (Active) proteinLIF 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Leukemia Inhibitory Factor (LIF) (AA 23-202) protein (His-GST)LIF 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90% by SDS-PAGE SDS
Leukemia Inhibitory Factor (LIF) (AA 23-202) protein (His tag)LIF 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % by SDS-PAGE gel analyses WB, ELISA
Leukemia Inhibitory Factor (LIF) (AA 24-203) protein (His tag)LIF 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Leukemia Inhibitory Factor (LIF) (AA 23-202) (Active) protein (GST tag)LIF 宿主: 人, 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % (SDS-PAGE) SDS Active
Leukemia Inhibitory Factor (LIF) (AA 1-202) protein (GST tag)LIF 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Leukemia Inhibitory Factor (LIF) proteinLIF 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by SDS-PAGE
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应用备注
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Application Note: Leukemia Inhibitory Factor Recombinant Protein has been tested by biological activity and is suitable as a control for polyclonal or monoclonal anti-Leukemia Inhibitory Factor in immunological assays.
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 5 μL (5-50 μL)
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浓度
- 0.1 mg/mL
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缓冲液
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Buffer: 0.1 % Trifluoroacetic acid
Stabilizer: None
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储存液
- Without preservative
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
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有效期
- 6 months
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- LIF (Leukemia Inhibitory Factor (LIF))
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别名
- LIF
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背景
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Synonyms: Differentiation-stimulating factor (D factor), Leukocyte Inhibitory Factor, Melanoma-derived LPL inhibitor (MLPLI)
Background: Human Leukemia Inhibitory Factor (LIF) is member of the IL-6 family and is made by a variety of tissues. Human LIF signals through gp130 to activate STAT3 in human ESCs . In mouse ESCs, LIF promotes self-renewal and pluripotency, but in human cells there is no indication that LIF has the same function. Instead, FGF-basic (FGF-2) and Activin A are used to promote self-renewal and pluripotent potential. Recombinant human LIF is a non-glycosylated protein, containing 181 amino acids, with a molecular weight of 19.9 kDa.
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UniProt
- P15018
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途径
- JAK/STAT Signaling, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Stem Cell Maintenance, Growth Factor Binding
抗原
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