Quick Overview for IL-9 蛋白 (ABIN6700172)
抗原
See all IL-9 (IL9) 蛋白蛋白类型
宿主
资源
应用范围
纯度
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Supplier Product No.
- 009-001-b96
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Supplier
- Rockland
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原理
- IL-9 Recombinant Human Protein
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纯化方法
- Purity was determined to be greater than 98% as determined by analysis by RP-HPLC and by reducing and non-reducing SDS- PAGE, against known standard.
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内毒素水平
- Measured by LAL is < 0.01 ng/μg or < 0.1 EU/μg protein.
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Biological Activity Comment
- IL-9 is fully biologically active when compared to a standard. The ED50 as determined by the dose-dependent proliferation of human MO7e cells is < 0.5 ng/ml.
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Interleukin 9 (IL9) (AA 19-144) protein (Fc Tag)
IL9 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 9 (IL9) (AA 19-144) protein (His tag)IL9 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 9 (IL9) (AA 19-144) protein (Fc Tag)IL9 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 9 (IL9) (AA 19-144) protein (His tag)IL9 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 9 (IL9) (AA 19-144) protein (Fc Tag)IL9 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Interleukin 9 (IL9) (AA 19-144) protein (His tag)IL9 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Interleukin 9 (IL9) (AA 1-144) protein (GST tag)IL9 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
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应用备注
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Application Note: IL-9 protein has been tested by SDS-PAGE and is suitable as a control for polyclonal or monoclonal anti-IL-9 in immunological assays.
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 10 μL (10-100 μL)
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缓冲液
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Buffer: 0.01 M Sodium Phosphate, pH 7.5
Stabilizer: None
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储存液
- Without preservative
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) . For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
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有效期
- 6 months
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- IL-9 (IL9) (Interleukin 9 (IL9))
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别名
- IL9
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背景
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Synonyms: Interleukin 9 cytokine, IL-9
Background: Interleukin (IL-9) is known to regulate many cell types involved in T- helper type 2 responses classically associated with asthma, including B- and T- lymphocytes, mast cells, eosinophils and epithelial cells. Growing evidence obtained from human genomic analysis and antigen-challenged transgenic mice suggests that IL-9 is a candidate factor in immunoglobulin E (IgE) production and thus is thought to be associated with bronchial inflammation and bronchial hyperresponsiveness (BHR). Recombinant Human IL-9 produced in E.coli is a single, non-glycosylated single polypeptide chain containing 127 amino acids and having a molecular mass of 14,004 Daltons.
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UniProt
- Q6NZ75
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途径
- JAK/STAT Signaling
抗原
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