Quick Overview for IL-27 蛋白 (ABIN6700106)
抗原
See all IL-27 (IL27) 蛋白蛋白类型
宿主
资源
应用范围
纯度
质量等级
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Supplier Product No.
- 010-f01-b54-0100
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Supplier
- Rockland
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原理
- Mouse Interleukin-27 (p28) Recombinant Protein (Animal Free)
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纯化方法
- The p28 subunit of IL-27 is produced with no animal-derived raw products, animal free equipment and animal free protocols. Purity was determined to be greater than 97% as determined by reducing and non-reducing SDS-PAGE.
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内毒素水平
- Measured by LAL is typically ≤ 1 EU/μg protein (with a 50% confidence range).
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Biological Activity Comment
- Mouse p28 biological activity was measured via dose-dependent inhibition of TGFß and IL-6-induced IL-17A expression in mouse CD4 splenocytes. 50ng/ml of Mouse p28 is capable of inhibiting >25% of IL-17A expression in this assay. no acceptance criteria limits established.
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Interleukin 27 (IL27) (AA 1-243) protein (GST tag)
IL27 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Interleukin 27 (IL27) protein (His tag)IL27 宿主: 小鼠 宿主: 人细胞 Recombinant > 90 % as determined by SDS-PAGE
Interleukin 27 (IL27) (AA 177-243) protein (GST tag)IL27 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
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应用备注
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Application Note: Interleukin-27 Recombinant Protein has been tested by SDS-PAGE and is suitable as a control for polyclonal or monoclonal anti-Interleukin-27 in immunological assays. Buffer Formulation: 10 mM sodium bicarbonate, pH 8.5.
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 100 μL
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缓冲液
- Buffer Formulation: 10 mM sodium bicarbonate, pH 8.5.
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储存液
- Without preservative
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
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有效期
- 6 months
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- IL-27 (IL27) (Interleukin 27 (IL27))
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别名
- Il27
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背景
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Synonyms: IL-30, P28, IL-27 p28 subunit
Background: The p28 subunit of IL-27 (IL-27/p28), also known as Interleukin-30 (IL-30), is a member of the IL-12 family of cytokines. When combined with EBI3 (Epstein-Barr virus induced gene 3), p28 forms a heterodimer known as IL-27. p28 is thought to be a pro-inflammatory cytokine inducing immuno-modulatory effects, but the details of these effects are currently unclear and research is focused on delineating its specific biological functions. Recombinant mouse p28 is a non-glycosylated protein, containing 207 amino acids, with a molecular weight of 23.7 kDa.
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UniProt
- Q8K3I6
抗原
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