Quick Overview for IL-2 蛋白 (ABIN6700068)
抗原
See all IL-2 (IL2) 蛋白蛋白类型
宿主
资源
应用范围
纯度
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Supplier Product No.
- 010-001-v27-0020
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Supplier
- Rockland
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原理
- Mouse Interleukin-2 Recombinant Protein
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纯化方法
- Interleukin-2 purity was determined to be greater than 98% as determined by HpLC, analysis by UV-Spectroscopy at 280nm, and by reducing and non-reducing SDS-pAGE.
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内毒素水平
- Measured by LAL is typically ≤ 1 EU/μg protein.
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Biological Activity Comment
- The activity is determined by its ability to induce the proliferation of CTLL-2 cells and is typically 0.1-0.4 ng/mL.
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Interleukin 2 (IL2) (AA 21-153) protein
IL2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 2 (IL2) protein (His tag)IL2 宿主: 人 宿主: HEK-293 Cells Recombinant Greater than 95 % as determined by reducing SDS-PAGE.
Interleukin 2 (IL2) (AA 21-169) protein (His-Avi Tag)IL2 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 2 (IL2) (AA 21-169) protein (His-Avi Tag,Biotin)IL2 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Interleukin 2 (IL2) (AA 21-153) protein (mFc Tag)IL2 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Interleukin 2 (IL2) (Active) proteinIL2 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Interleukin 2 (IL2) (Active) proteinIL2 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by reducing SDS-PAGE. Active
Interleukin 2 (IL2) (AA 21-153) (Active) proteinIL2 宿主: 人 宿主: CHO Cells Recombinant > 95 % as analyzed by SDS-PAGE. Active
Interleukin 2 (IL2) (AA 21-153) protein (His tag)IL2 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Interleukin 2 (IL2) (AA 21-153) (Active) protein (Fc Tag,AVI tag,Biotin)IL2 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
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应用备注
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Application Note: Mouse Interleukin-2 Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Mouse Interleukin-2 in immunological assays. Lyophilized in 0.01M sodium citrate, pH 4.0.
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 20 μL (20-200 μL)
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缓冲液
- Lyophilized in 0.01M sodium citrate, pH 4.0.
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储存液
- Without preservative
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储存条件
- -20 °C
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储存方法
- Store vial at -20° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
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有效期
- 6 months
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- IL-2 (IL2) (Interleukin 2 (IL2))
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别名
- IL2
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背景
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Synonyms: T cell growth factor, TCGF, Aldesleukin
Background: Interleukin-2 (IL-2) is an immuno-modulatory cytokine produced by stimulated T cells. IL-2 signals through the IL-2R receptor to induce proliferation of activated T cells. IL-2 also stimulates proliferation and differentiation of B cells, natural killer cells, monocytes and macrophages. Recombinant mouse IL-2 is a non-glycosylated protein, containing 149 amino acids, with a molecular weight of 17.2 kDa.
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UniProt
- P04351
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途径
- JAK/STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation
抗原
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