IL17F 蛋白
Quick Overview for IL17F 蛋白 (ABIN6700053)
抗原
See all IL17F 蛋白蛋白类型
宿主
资源
应用范围
纯度
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Supplier Product No.
- 009-001-b31
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Supplier
- Rockland
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原理
- IL-17F Human Recombinant Protein
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纯化方法
- Purity was determined to be approximately 97% by analysis by RP-HPLC and by reducing and non-reducing SDS-PAGE.
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内毒素水平
- Measured by LAL is < 0.01 ng/μg or < 0.1 EU/μg protein.
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Biological Activity Comment
- The ED50 of recombinant human IL-17F, as determined by induction of IL-6 in NIH3T3 cells, is 10.18 ng/mL.
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应用备注
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Application Note: IL-17F protein has been tested by SDS-PAGE and is suitable as a control for polyclonal or monoclonal anti-IL-17F in immunological assays.
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 25 μL (25-250 μL)
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浓度
- 0.1 mg/mL
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缓冲液
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Buffer: 0.1 % Trifluoroacetic acid
Stabilizer: None
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储存液
- Without preservative
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) . For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
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有效期
- 6 months
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- IL17F (Interleukin 17F (IL17F))
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别名
- IL17F
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背景
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Synonyms: Interleukin-17F cytokine, IL-17F, Interleukin-24, IL-24, Cytokine ML-1
Background: Recombinant Human Interleukin-17-F is a non-glycosylated protein consisting of two 134 amino acid polypeptide chains that are cysteine linked homodimeric polypeptide chains with a molecular weight of 30 kDa. Homodimeric interleukin-17F is a member of the IL-17 family of six homodimeric proteins (IL-17A-17F). Activity studies reveal IL-17A to be the most potent, followed by IL-17A/F and IL-17F 100 fold lower than IL-17A.
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UniProt
- Q96PD4
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途径
- Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity
抗原
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