Quick Overview for IFNG1-2 蛋白 (ABIN6699981)
抗原
蛋白类型
宿主
资源
应用范围
纯度
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Supplier Product No.
- 012-001-v20-0020
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Supplier
- Rockland
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原理
- Rat Interferon gamma Recombinant Protein
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纯化方法
- Interferon gamma purity was determined to be greater than 95% as determined by HpLC, analysis by UV-Spectroscopy at 280nm, and by reducing and non-reducing SDS-pAGE.
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内毒素水平
- Measured by LAL is typically ≤ 1 EU/μg protein.
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Biological Activity Comment
- The activity is determined in a viral challenge assay using EMC virus on L929 and is typically less than 0.1 ng/mL.
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Interferon, gamma 1-2 (IFNG1-2) protein
IFNG1-2 宿主: 大鼠 宿主: 大肠杆菌（E. Coli） Recombinant 95,00% SDS
Interferon, gamma 1-2 (IFNG1-2) proteinIFNG1-2 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant 95,00% SDS
Interferon, gamma 1-2 (IFNG1-2) proteinIFNG1-2 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant 95,00% SDS
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应用备注
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Application Note: Interferon-gamma Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Interferon-gamma in immunological assays. Lyophilized in 10 mM sodium phosphate, 100 mM sodium chloride, pH 7.5.
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 20 μL (20-200 μL)
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浓度
- 0.1 mg/mL
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缓冲液
- Lyophilized in 10 mM sodium phosphate, 100 mM sodium chloride, pH 7.5.
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储存液
- Without preservative
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
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有效期
- 6 months
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- IFNG1-2 (Interferon, gamma 1-2 (IFNG1-2))
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别名
- INF gamma
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背景
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Synonyms: Type II interferon, T cell interferon, Immune Interferon, MAF
Background: Interferon gamma, IFNγ, is a major immune-modulating molecule produced mainly by T cells and natural killer cells activated by antigens, mitogens or alloantigens. Most immune cells express IFNγ receptors and respond to IFNγ-induced signaling by up-regulating MHC class I expression. Recombinant rat IFNγ is a non-glycosylated protein, containing 135 amino acids, with a molecular weight of 15.6 kDa.
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UniProt
- P01581
抗原
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