HSPA1AB protein (GST tag)
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北京 101111
Quick Overview for HSPA1AB protein (GST tag) (ABIN6699933)
抗原
蛋白类型
宿主
资源
应用范围
纯度
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标记
- GST tag
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Supplier Product No.
- 009-001-f63s
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Supplier
- Rockland
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原理
- HSP70 recombinant protein-GST fusion protein
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纯化方法
- Recombinant full-length human HSP70 was expressed in E. coli cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >90% by densitometry.
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应用备注
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Application Note: HSP70 Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol. HSP70 Protein is suitable for use in Western Blot and ATPase Assay. Expect a band approximately ~96 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.
Western Blot Dilution: User Optimized
Other: ATPase Assay-User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.2 μg/μL
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缓冲液
- HSP70 Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.
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储存条件
- -80 °C
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储存方法
- Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
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有效期
- 12 months
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- HSPA1AB
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背景
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Synonyms: HSPA1A, HSP72, HSPA1, HSPA1B, HSP70-1, Heat shock 70 kDa protein 1A/1B , Heat shock 70 kDa protein 1/2, HSP70-1/HSP70-2, HSP70.1/HSP70.2
Background: HSP70 is a member of the heat shock protein family and is synthesized by cells of many organisms in response to stress (1). HSP70 is found mostly but not exclusively in the nucleus of unstressed cells. For several hours after a short heat shock, however, it is strongly concentrated in nucleoli (2). Nucleoli are transiently damaged by such a heat shock: their morphology changes and assembly and export of ribosomes is blocked for several hours. HSP70 helps to stabilize the morphological changes of the nucleoli. HSP70 Protein is ideal for investigators involved in Signaling Proteins, Cell Stress & Chaperone Proteins, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, Cellular Stress, and Inflammation research.
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NCBI登录号
- NM_005345
抗原
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