Quick Overview for GDF5 蛋白 (ABIN6699913)
抗原
See all GDF5 蛋白蛋白类型
宿主
资源
应用范围
纯度
-
-
Supplier Product No.
- 009-001-u94-0050
-
Supplier
- Rockland
-
原理
- Human Growth and Differentiation Factor-5 Recombinant Protein
-
纯化方法
- Growth and Differentiation Factor-5 purity was determined to be greater than 98% as determined by analysis by HpLC, UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-pAGE.
-
内毒素水平
- Measured by LAL is typically ≤ 1 EU/μg protein.
-
Biological Activity Comment
- The activity is determined by the ability to induce alkaline phosphatase activity in ATDC5 cells is typically 0.1-1 μg/mL.
-
-
Growth Differentiation Factor 5 (GDF5) (AA 382-501) protein (His-IF2DI Tag)
GDF5 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Growth Differentiation Factor 5 (GDF5) (AA 376-495) protein (His tag)GDF5 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Growth Differentiation Factor 5 (GDF5) proteinGDF5 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by reducing SDS-PAGE.
Growth Differentiation Factor 5 (GDF5) (AA 28-127) protein (GST tag)GDF5 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Growth Differentiation Factor 5 (GDF5) (AA 376-495) protein (His tag)GDF5 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % by SDS - PAGE SDS
Growth Differentiation Factor 5 (GDF5) proteinGDF5 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 98,00% SDS
-
-
应用备注
-
Application Note: Growth and Differentiation Factor-5 Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Growth and Differentiation Factor-5 in immunological assays.
Other: User Optimized
-
限制
- 仅限研究用
-
-
-
状态
- Lyophilized
-
溶解方式
-
Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 50μL
-
缓冲液
-
Buffer: 0.1 % Trifluoroacetic acid
Stabilizer: None
-
储存液
- Without preservative
-
储存条件
- 4 °C,-20 °C
-
储存方法
- Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
-
有效期
- 6 months
-
-
- GDF5 (Growth Differentiation Factor 5 (GDF5))
-
别名
- GDF5
-
背景
-
Synonyms: Cartilage-derived morphogenetic protein 1
Background: Growth/Differentiation Factor 5 (GDF-5) is a growth factor that regulates cell proliferation and differentiation in embryonic and adult tissues. GDF-5 is part of the TGF family of proteins and is closely related to the BMP family of proteins. Recombinant human GDF-5 is a non-glycosylated homodimer, containing two 120 amino acids chains, with a total molecular weight of 27.4 kDa. To enable bacterial expression the N-terminal sequence of Ala-Pro-Leu-Thr was replaced with a Lys.
-
UniProt
- P43026
抗原
-
