Quick Overview for GM-CSF 蛋白 (ABIN6699902)
抗原
See all GM-CSF (CSF2) 蛋白蛋白类型
宿主
资源
应用范围
纯度
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Supplier Product No.
- 012-001-v12-0005
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Supplier
- Rockland
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原理
- Rat Granulocyte Macrophage-Colony Stimulating Factor Recombinant Protein
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纯化方法
- Granulocyte Macrophage-Colony Stimulating Factor purity was determined to be greater than 97% as determined by UV spectroscopy at 280 nm, Rp-HpLC calibrated against a known standard, and Quantitation against a known standard via reducing and non-reducing SDS-pAGE gels.
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内毒素水平
- Measured by LAL is typically ≤ 1 EU/μg protein.
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Biological Activity Comment
- The activity is determined by the dose-dependant proliferation of the mouse FDC-P1 cell line and is typically less than 100 pg/mL.
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Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (AA 18-144) protein
CSF2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (Active) protein (His tag)CSF2 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by SDS-PAGE Active
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (Active) proteinCSF2 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by SDS-PAGE Active
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (AA 18-144) protein (Fc Tag)CSF2 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (AA 18-141) (Active) proteinCSF2 宿主: 小鼠 宿主: CHO Cells Recombinant > 95 % as analyzed by SDS-PAGE and HPLC. Active
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) protein (His tag)CSF2 宿主: 大鼠 宿主: 人细胞 Recombinant > 95 % as determined by SDS-PAGE
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (AA 1-144) protein (GST tag)CSF2 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (AA 18-144) (Active) protein (His tag)CSF2 宿主: 人 宿主: HEK-293 Cells > 95 % AcA, CC Active
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应用备注
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Application Note: Granulocyte Macrophage Colony Stimulating Factor Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Granulocyte Macrophage Colony Stimulating Factor in immunological assays. Lyophilized in 20 mM sodium bicarbonate buffer, pH 8.5.
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 5 μL (5-50 μL)
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缓冲液
- Lyophilized in 20 mM sodium bicarbonate buffer, pH 8.5.
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储存液
- Without preservative
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
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有效期
- 6 months
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- GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))
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别名
- csf2
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背景
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Synonyms: Colony-stimulating factor (CSF), Pluripoietin-α, MGI1GM
Background: Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) is hematopoietic factor produced by endothelial cells, monocytes, fibroblasts and T cells in response to a number of inflammatory mediators. GM-CSF is able to stimulate the production of neutrophilic granulocytes, macrophages, and mixed granulocyte-macrophage colonies from bone marrow cells. GM-CSF can also stimulate some functional activities in mature granulocytes and macrophages. Human and mouse GM-CSF show no cross-reactivity. Recombinant rat GM-CSF is a non-glycosylated protein, containing 128 amino acids, with a molecular weight of 14.6 kDa.
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UniProt
- P48750
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途径
- JAK/STAT Signaling, Cellular Response to Molecule of Bacterial Origin
抗原
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