Quick Overview for GM-CSF 蛋白 (ABIN6699896)
抗原
See all GM-CSF (CSF2) 蛋白蛋白类型
宿主
资源
应用范围
纯度
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Supplier Product No.
- 010-001-314
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Supplier
- Rockland
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原理
- GM-CSF Mouse Recombinant Protein
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纯化方法
- Purity greater than 95% as determined by analysis by RP-HPLC and reducing and non- reducing SDS-PAGE.
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内毒素水平
- Measured by LAL is typically ≤ 1 EU/μg protein.
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Biological Activity Comment
- GM-CSF activity is determined by the dose-dependent proliferation of mouse FDCP-1 cell line and is typically less than 10 pg/mL.
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Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (AA 18-144) protein
CSF2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (Active) protein (His tag)CSF2 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by SDS-PAGE Active
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (Active) proteinCSF2 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by SDS-PAGE Active
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (AA 18-144) protein (Fc Tag)CSF2 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (AA 18-141) (Active) proteinCSF2 宿主: 小鼠 宿主: CHO Cells Recombinant > 95 % as analyzed by SDS-PAGE and HPLC. Active
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) protein (His tag)CSF2 宿主: 大鼠 宿主: 人细胞 Recombinant > 95 % as determined by SDS-PAGE
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (AA 1-144) protein (GST tag)CSF2 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2) (AA 18-144) (Active) protein (His tag)CSF2 宿主: 人 宿主: HEK-293 Cells > 95 % AcA, CC Active
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应用备注
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Application Note: GM-CSF protein has been tested by SDS-PAGE and is suitable as a control for polyclonal or monoclonal anti-GM-CSF in immunological assays. Lyophilized in 10 mM acetic Acid.
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 20 μL (20-200 μL)
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浓度
- 0.2 mg/mL
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缓冲液
- Lyophilized in 10 mM acetic Acid.
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储存液
- Without preservative
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) . For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
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有效期
- 6 months
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- GM-CSF (CSF2) (Colony Stimulating Factor 2 (Granulocyte-Macrophage) (CSF2))
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别名
- Csf2
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背景
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Synonyms: Granulocyte Macrophage Colony Stimulating Factor, Granulocyte Macrophage-CSF, Granulocyte-macrophage colony-stimulating factor, GM-CSF cytokine, Colony-stimulating factor, CSF, CSF2, Sargramostim, Molgramostin
Background: M-GM-CSF is produced in response to a number of inflammatory mediators by mesenchymal cells present in the hemopoietic environment and at peripheral sites of inflammation. GM-CSF is able to stimulate the production of neutrophilic granulocytes, macrophages, and mixed granulocyte-macrophage colonies from bone marrow cells and can stimulate the formation of eosinophil colonies from fetal liver progenitor cells. GM-CSF can also stimulate some functional activities in mature granulocytes and macrophages. GM-CSF receptors shows significant homologies with other receptors for hematopoietic growth factors, including IL2 -beta, IL-3, IL-6, IL-7, EPO and the Prolactin receptors. Recombinant mouse GM- CSF produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 125 amino acids and having a molecular mass of 14,285.35 Daltons.
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UniProt
- Q14AD9
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途径
- JAK/STAT Signaling, Cellular Response to Molecule of Bacterial Origin
抗原
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