Follistatin Protein (FST)
Quick Overview for Follistatin Protein (FST) (ABIN6699882)
抗原
See all Follistatin (FST) 蛋白蛋白类型
宿主
资源
应用范围
纯度
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Supplier Product No.
- 010-001-u88-0005
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Supplier
- Rockland
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原理
- Mouse Follistatin Recombinant Protein
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纯化方法
- Follistatin purity was determined to be greater than 90% as determined by analysis by reducing and non-reducing SDS-pAGE.
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内毒素水平
- Measured by LAL is typically ≤ 1 EU/μg protein.
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Biological Activity Comment
- The activity is determined by the dose-dependent neutralization of 7.5 ng/mL human Activin A. Complete neutralization is typically reached at less than 0.3 ug/mL.
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Follistatin (FST) (AA 30-344) protein (His tag)
FST 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Follistatin (FST) (AA 30-317) protein (His-IF2DI Tag)FST 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Follistatin (FST) (AA 1-344) protein (GST tag)FST 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Follistatin (FST) (Active) protein (Fc Tag)FST 宿主: 小鼠 宿主: CHO Cells Recombinant > 90 % as determined by SDS-PAGE Active
Follistatin (FST) (Active) protein (His tag)FST 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 98 % as determined by SDS-PAGE Active
Follistatin (FST) proteinFST 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 95,00% SDS
Follistatin (FST) proteinFST 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant 90,00% SDS
Follistatin (FST) proteinFST 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 95,00% SDS
Follistatin (FST) proteinFST 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant 90,00% SDS
Follistatin (FST) proteinFST 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 95,00% SDS
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应用备注
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Application Note: Follistatin Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Follistatin in immunological assays. Buffer Formulation: 10 mM sodium phosphate, 50 mM sodium chloride, pH 7.5.
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 5 μL (5-50 μL)
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缓冲液
- Buffer Formulation: 10 mM sodium phosphate, 50 mM sodium chloride, pH 7.5.
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储存液
- Without preservative
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
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有效期
- 6 months
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- Follistatin (FST)
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别名
- Fst
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背景
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Synonyms: FS, activin-binding protein, FSH-suppressing protein (FSP)
Background: Follistatin is an autocrine acting protein that is expressed by many tissues, but at noteably higher levels in the ovary and skin. Follistatin functions to negatively regulate the signaling of a wide variety of TGF-beta family members (activin, BMPs, myostatin, GDF-11 and TGF-beta 1). Mechanistically, follistatin works as an antagonist by complexing with TGF-beta family members to prevent them from interacting with their signaling receptors. Recombinant mouse follistatin is a non-glycosylated protein, containing 289 amino acids, with a molecular weight of 31.6 kDa.
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UniProt
- P47931
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途径
- Negative Regulation of Hormone Secretion
抗原
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