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Cyclin H Protein (CCNH) (GST tag)

This Recombinant Cyclin H protein is expressed in Insect cells (Sf9).
Rockland
产品编号 ABIN6699745
Supplier Product No.: 009-001-r80s
发货至: 中国
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Quick Overview for Cyclin H Protein (CCNH) (GST tag) (ABIN6699745)

抗原

See all Cyclin H (CCNH) 蛋白
Cyclin H (CCNH)

蛋白类型

Recombinant

宿主

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资源

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Insect cells (Sf9)

应用范围

Western Blotting (WB)

纯度

>90%
  • 标记

    This Cyclin H protein is labelled with GST tag.

    Supplier Product No.

    009-001-r80s

    Supplier

    Rockland

    原理

    CyclinH recombinant protein-GST fusion protein

    纯化方法

    Recombinant full length human CyclinH was expressed in insect cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >90% by densitometry.
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  • 应用备注

    Application Note: CyclinH Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol. CyclinH Protein is suitable for use in Western Blot. Expect a band approximately ~ 39 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

    Western Blot Dilution: User Optimized

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.1 μg/μL

    缓冲液

    CyclinH Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.

    储存条件

    -80 °C

    储存方法

    Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

    有效期

    12 months
  • 抗原

    Cyclin H (CCNH)

    别名

    CCNH

    背景

    Synonyms: CCNH, CAK, p34, p37, Cyclin-H, MO15-associated protein

    Background: Cyclin H is related to the highly conserved cyclin family. Cyclin family members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Cyclin H forms a complex with CDK7 kinase and the ring finger protein MAT1 which is able to phosphorylate CDK2 and CDC2 kinases and thus act as a CDK-activating kinase (CAK) (1). Cdk-activating kinase complex is a component of human transcription factor TFIIH and plays an important role in the processes of transcription, DNA repair, and cell cycle progression (2). Cyclin H Protein is ideal for investigators involved in Signaling Proteins, Cell Cycle Proteins, Cancer, and Cell Cycle research.

    NCBI登录号

    NM_001239

    途径

    Cell Division Cycle, Mitotic G1-G1/S Phases, M Phase
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