Cyclin H Protein (CCNH) (GST tag)
Quick Overview for Cyclin H Protein (CCNH) (GST tag) (ABIN6699745)
抗原
See all Cyclin H (CCNH) 蛋白蛋白类型
宿主
资源
应用范围
纯度
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标记
- This Cyclin H protein is labelled with GST tag.
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Supplier Product No.
- 009-001-r80s
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Supplier
- Rockland
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原理
- CyclinH recombinant protein-GST fusion protein
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纯化方法
- Recombinant full length human CyclinH was expressed in insect cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >90% by densitometry.
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Cyclin H (CCNH) (AA 1-323) protein (His tag)
custom-made CCNH 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Cyclin H (CCNH) (AA 1-323) protein (His tag)custom-made CCNH 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Cyclin H (CCNH) (AA 1-323) protein (GST tag)CCNH 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
Cyclin H (CCNH) protein (Myc-DYKDDDDK Tag)CCNH 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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应用备注
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Application Note: CyclinH Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol. CyclinH Protein is suitable for use in Western Blot. Expect a band approximately ~ 39 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.
Western Blot Dilution: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.1 μg/μL
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缓冲液
- CyclinH Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.
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储存条件
- -80 °C
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储存方法
- Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
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有效期
- 12 months
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- Cyclin H (CCNH)
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别名
- CCNH
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背景
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Synonyms: CCNH, CAK, p34, p37, Cyclin-H, MO15-associated protein
Background: Cyclin H is related to the highly conserved cyclin family. Cyclin family members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Cyclin H forms a complex with CDK7 kinase and the ring finger protein MAT1 which is able to phosphorylate CDK2 and CDC2 kinases and thus act as a CDK-activating kinase (CAK) (1). Cdk-activating kinase complex is a component of human transcription factor TFIIH and plays an important role in the processes of transcription, DNA repair, and cell cycle progression (2). Cyclin H Protein is ideal for investigators involved in Signaling Proteins, Cell Cycle Proteins, Cancer, and Cell Cycle research.
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NCBI登录号
- NM_001239
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途径
- Cell Division Cycle, Mitotic G1-G1/S Phases, M Phase
抗原
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