Cyclin C Protein (CCNC) (GST tag)
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Quick Overview for Cyclin C Protein (CCNC) (GST tag) (ABIN6699737)
抗原
See all Cyclin C (CCNC) 蛋白蛋白类型
宿主
资源
应用范围
纯度
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标记
- This Cyclin C protein is labelled with GST tag.
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Supplier Product No.
- 009-001-r77s
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Supplier
- Rockland
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原理
- CyclinC recombinant protein-GST fusion protein
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纯化方法
- Recombinant full-length human CyclinC was expressed by baculovirus in Sf9 insect cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >70% by densitometry.
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Cyclin C (CCNC) (AA 1-283) protein (His tag)
custom-made CCNC 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Cyclin C (CCNC) (AA 1-283) protein (His tag)custom-made CCNC 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Cyclin C (CCNC) (AA 1-283) protein (GST tag)CCNC 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
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应用备注
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Application Note: CyclinC Protein is stored in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol. CyclinC Protein is suitable for use in Western Blot. Expect a band approximately ~ 55 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.
Western Blot Dilution: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.05 μg/μL
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缓冲液
- CyclinC Protein is stored in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.
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储存条件
- -80 °C
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储存方法
- Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
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有效期
- 12 months
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- Cyclin C (CCNC)
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别名
- CCNC
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背景
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Synonyms: CCNC, CycC, Cyclin-C, SRB11 homolog, hSRB11
Background: Cyclin C is a member of the cyclin family of proteins and interacts with the cyclin-dependent kinase 8 (CDK8) and induces the phosphorylation of the carboxy-terminal domain of the large subunit of RNA polymerase II. The mRNA levels of Cyclin C fluctuate during the cell cycle and peak at the G1 phase. Cyclin C acts as a critical regulator of the G0/G1 transition of human Hematopoietic stem cells (1). Cyclin C can also combine with CDK3 to stimulate Rb phosphorylation at S807/811 during the G0/G1 transition, and this phosphorylation is required for cells to exit G0 efficiently (2). Cyclin C Protein is ideal for investigators involved in Signaling Proteins, Cell Cycle Proteins, Cancer, and Cell Cycle research.
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NCBI登录号
- NM_005190
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途径
- Cell Division Cycle, Regulation of Lipid Metabolism by PPARalpha
抗原
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