Cofilin Protein (His tag)
Quick Overview for Cofilin Protein (His tag) (ABIN6699707)
抗原
See all Cofilin (CFL1) 蛋白蛋白类型
宿主
资源
应用范围
纯度
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标记
- This Cofilin protein is labelled with His tag.
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Supplier Product No.
- 009-001-r72s
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Supplier
- Rockland
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原理
- Cofilin 1 recombinant protein-HIS Epitope
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纯化方法
- Recombinant full-length human Cofilin 1 was expressed in E. coli cells using an N-Terminal his epitope. The purity was determined to be >95% by densitometry.
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Cofilin 1 (CFL1) (AA 2-166) protein (His tag)
CFL1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Cofilin 1 (CFL1) (AA 1-166) protein (GST tag)CFL1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Cofilin 1 (CFL1) (AA 1-166) protein (His tag)CFL1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS - PAGE SDS
Cofilin 1 (CFL1) protein (Myc-DYKDDDDK Tag)CFL1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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应用备注
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Application Note: Cofilin 1 Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25 % glycerol. Cofilin 1 Protein is suitable for use in Western Blot. Expect a band approximately ~ 23 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.
Western Blot Dilution: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.2 μg/μL
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缓冲液
- Cofilin 1 Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25 % glycerol.
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储存条件
- -80 °C
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储存方法
- Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
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有效期
- 12 months
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- Cofilin (CFL1) (Cofilin 1 (CFL1))
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别名
- CFL1
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背景
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Synonyms: CFL1, CFL, Cofilin-1, 18 kDa phosphoprotein, p18, Cofilin, non-muscle isoform
Background: Cofilin 1 (non-muscle), also known as CFL1, is a widely distributed intracellular actin-modulating protein that binds and depolymerizes filamentous F-actin and inhibits the polymerization of monomeric G-actin in a pH -dependent manner (1). It is involved in the translocation of actin-cofilin complex from cytoplasm to nucleus (2). CFL1 processed proteins are most probably produced by HIV-1 protease digestion. Cofilin 1 Protein is ideal for investigators involved in Signaling Proteins, Microtubule/Actin Associated Proteins, Cancer, Cardiovascular Disease, Invasion/Metastasis, and Neurobiology research.
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NCBI登录号
- NM_005507
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途径
- Regulation of Cell Size, Tube Formation, Protein targeting to Nucleus, CXCR4-mediated Signaling Events
抗原
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