Quick Overview for CX3CL1 蛋白 (ABIN6699702)
抗原
See all CX3CL1 蛋白蛋白类型
宿主
资源
纯度
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Supplier Product No.
- 009-001-h49-0005
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Supplier
- Rockland
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原理
- Human Fractalkine (CX3CL1) Recombinant Protein
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纯化方法
- Fractalkine (CX3CL1) purity was determined to be greater than 98% as determined by analysis by HpLC, UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-pAGE.
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内毒素水平
- Measured by LAL is typically ≤ 1 EU/μg protein.
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Biological Activity Comment
- The activity is determined by the dose dependent chemotaxis of human PBMCs and is typically starting between 10-100 ng/mL.
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Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1) (AA 25-336) protein (His tag)
CX3CL1 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1) (AA 25-341) protein (His tag)CX3CL1 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1) (AA 25-341) protein (His tag)CX3CL1 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1) (AA 25-336) protein (His-Avi Tag,Biotin)CX3CL1 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1) (Active) proteinCX3CL1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1) (AA 25-100) protein (His tag)CX3CL1 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1) (AA 76-182) protein (His tag)CX3CL1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1) (AA 1-397) protein (GST tag)CX3CL1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1) (AA 1-236) protein (GST tag)CX3CL1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1) (AA 351-449) protein (GST tag)CX3CL1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
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应用备注
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Other: User Optimized
Application_Note: Fractalkine Recombinant Protein has been tested by biological activity and is suitable as a control for polyclonal or monoclonal anti-Fractalkine in immunological assays.
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说明
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Suggested_Applications: Cellular Assay
Other_Performance_Data:
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 5 μL (5-50 μL)
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缓冲液
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Buffer: 0.1 % Trifluoroacetic acid
Stabilizer: None
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储存液
- Without preservative
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
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有效期
- 6 months
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- CX3CL1 (Chemokine (C-X3-C Motif) Ligand 1 (CX3CL1))
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别名
- CX3CL1
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背景
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Synonyms: C-X3-C motif chemokine 1, CX3C membrane-anchored chemokine, Neurotactin, Small-inducible cytokine D1
Background: Fractalkine, also known as CX3CL1, is an atypical chemokine that was the first of a fourth chemokine motif (CX3C). It is thought to function as a T cell and monocyte chemotractant and is produced by non-haemopoietic cells. Fractalkine is made in a soluble and membrane bound form in activated endothelial cells which is thought to promote adhesion of leukocytes. Recombinant human Fractalkine is a non-glycosylated protein, containing 76 amino acids, with a molecular weight of 8.6 kDa.
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UniProt
- P78423
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途径
- Synaptic Membrane
抗原
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