Quick Overview for CCL19 蛋白 (ABIN6699624)
抗原
See all CCL19 蛋白蛋白类型
宿主
资源
应用范围
纯度
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Supplier Product No.
- 009-001-c21
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Supplier
- Rockland
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原理
- MIP-3ß Human Recombinant Protein
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纯化方法
- Recombinant protein corresponds to amino acids 22 to 98 of mature human MIP-3ß. Purity is greater than 98% as determined by reducing and non-reducing SDS-PAGE and by RP- HPLC, against known standard.
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内毒素水平
- Measured by LAL is < 0.01 ng/μg or < 0.1 EU/μg protein.
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Biological Activity Comment
- Macrophage Inflammatory Protein 3ß is fully biologically active when compared to standard. The activity is calculated by the ability to chemoattract human T cells using a concentration of 7 - 40 ng/ml.
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Chemokine (C-C Motif) Ligand 19 (CCL19) (AA 22-98) protein (T7 tag)
CCL19 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % by SDS - PAGE SDS
Chemokine (C-C Motif) Ligand 19 (CCL19) (AA 26-108) protein (His tag)CCL19 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Chemokine (C-C Motif) Ligand 19 (CCL19) (AA 22-98) (Active) proteinCCL19 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 97 % by SDS PAGE CMA Active
Chemokine (C-C Motif) Ligand 19 (CCL19) (AA 1-98) protein (GST tag)CCL19 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Chemokine (C-C Motif) Ligand 19 (CCL19) (AA 22-98) protein (Fc Tag)CCL19 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Chemokine (C-C Motif) Ligand 19 (CCL19) (AA 22-98) (Active) protein (Biotin)CCL19 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 97 % by SDS PAGE CMA Active
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应用备注
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Application Note: MIP-3ß Recombinant Protein has been tested by SDS-PAGE and is suitable as a control for polyclonal or monoclonal anti-MIP-3ß in immunological assays.
Other: User Optimized
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
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Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 20 μL (20-200 μL)
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浓度
- 0.1 mg/mL
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缓冲液
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Buffer: 0.1 % Trifluoroacetic acid
Stabilizer: None
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储存液
- Without preservative
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储存条件
- 4 °C,-20 °C
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储存方法
- Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) . For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
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有效期
- 6 months
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- CCL19 (Chemokine (C-C Motif) Ligand 19 (CCL19))
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别名
- CCL19
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背景
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Synonyms: C-C motif chemokine 19, Small-inducible cytokine A19, Macrophage inflammatory protein 3 beta, MIP-3-beta, MIP3B, Epstein-Barr virus-induced molecule 1 ligand chemokine, EBI1 ligand chemokine, ELC, Beta-chemokine exodus-3, CK beta-11, SCYA19
Background: The MIP-3ß (also known as SCYA19) member of the intercrine beta (chemokine CC) family may play a role not only in inflammatory and immunological responses, but also in normal lymphocyte recirculation and homing. May play an important role in trafficking of T-cells in thymus, and T-cell and B-cell migration to secondary lymphoid organs. It is secreted. It specifically binds to chemokine receptor CCR7. MIP3ß is expressed at high levels in the lymph nodes, thymus and appendix, and at intermediate levels seen in colon and trachea, while low levels found in spleen, small intestine, lung, kidney and stomach. Recombinant Human MIP-3b produced in E. coli is a single, non-glycosylated, polypeptide chain containing 77 amino acids and having a molecular mass of 8809 Daltons. The recombinant protein shows potent chemotactic activity for T-cells and B-cells but not for granulocytes and monocytes.
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UniProt
- Q99731
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途径
- Positive Regulation of Immune Effector Process
抗原
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