PPM1F Protein (GST tag)
-
- 抗原 See all PPM1F 蛋白
- PPM1F (Protein Phosphatase 1F (PP2C Domain Containing) (PPM1F))
- 蛋白类型
- Recombinant
-
宿主
- 人
-
资源
- 大肠杆菌（E. Coli）
- 标记
- This PPM1F protein is labelled with GST tag.
- 应用范围
- Western Blotting (WB)
- Supplier Product No.
- 009-001-r56s
- Supplier
- Rockland
- 原理
- CaMKPase recombinant protein-GST fusion protein
- 纯化方法
- Recombinant full-length human CaMKPase was expressed in E. coli cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >80% by densitometry.
- 纯度
- >80%
- Top Product
- Discover our top product PPM1F 蛋白
-
Protein Phosphatase 1F (PP2C Domain Containing) (PPM1F) (AA 1-454) protein (GST tag)
PPM1F 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
Protein Phosphatase 1F (PP2C Domain Containing) (PPM1F) (AA 1-454) protein (His tag)PPM1F 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Protein Phosphatase 1F (PP2C Domain Containing) (PPM1F) (AA 1-452) protein (His tag)custom-made PPM1F 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Protein Phosphatase 1F (PP2C Domain Containing) (PPM1F) (AA 1-454) protein (His tag)custom-made PPM1F 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Protein Phosphatase 1F (PP2C Domain Containing) (PPM1F) protein (Myc-DYKDDDDK Tag)PPM1F 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
-
- 应用备注
-
Application Note: CaMKPase Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol. CaMKPase Protein is suitable for use in Western Blot. Expect a band approximately ~ 74 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.
Western Blot Dilution: User Optimized
- 限制
- 仅限研究用
-
- 状态
- Liquid
- 浓度
- 0.2 μg/μL
- 缓冲液
- CaMKPase Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.
- 储存条件
- -80 °C
- 储存方法
- Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
- 有效期
- 12 months
-
- 抗原
- PPM1F (Protein Phosphatase 1F (PP2C Domain Containing) (PPM1F))
- 别名
- PPM1F (PPM1F 产品)
- 背景
-
Synonyms: PPM1F, FEM-2, hFEM-2, KIAA0015, POPX2, Protein phosphatase 1F, Ca(2+)/calmodulin-dependent protein kinase phosphatase, CaM-kinase phosphatase, CaMKPase, Partner of PIX 2, Protein fem-2 homolog, hFem-2
Background: CaMKPase is a member of the PP2C family of Ser/Thr protein phosphatases that dephosphorylate and regulate the multifunctional Ca2+/calmodulin-dependent protein kinases (CaMKs) (1). The multifunctional CaMKs mediate cellular responses induced by increases in second messenger Ca2+ and have been implicated in the control of synaptic transmission, gene transcription, cell growth and contraction of cardiac and smooth muscles (2). Overexpression of CaMKPase has been shown to mediate caspase-dependent apoptosis. CaMKPase can also interact with the Rho guanine nucleotide exchange factors (PIX) thereby blocking the effects of p21-activated kinase 1 (PAK1). CaMKPase Protein is ideal to investigators involved in Signaling Proteins, Cellular Proteins, Cardiovascular Disease, ERK/MAPK Pathway, Neurobiology, Phosphatases, and PKA/PKC Pathway research.
- NCBI登录号
- NM_014634
- 途径
- Positive Regulation of Endopeptidase Activity
-
