BAG1 Protein (GST tag)
Quick Overview for BAG1 Protein (GST tag) (ABIN6699543)
抗原
See all BAG1 蛋白蛋白类型
宿主
资源
应用范围
纯度
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标记
- This BAG1 protein is labelled with GST tag.
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Supplier Product No.
- 009-001-r46s
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Supplier
- Rockland
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原理
- BAG1 recombinant protein-GST fusion protein
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纯化方法
- Recombinant human BAG1 (72-end) was expressed in E. coli cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >85% by densitometry.
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!探索我们的预定义定制蛋白和定制蛋白服务！Product表达系统ConjugateOrigin价格从表达系统 HEK-293 CellsConjugate His tagOrigin Human价格从 115,504.49 ¥表达系统 Cell-free protein synthesis (CFPS)Conjugate Strep TagOrigin Human价格从 152,621.24 ¥您的项目需要进一步定制吗？联系我们，了解我们的定制蛋白解决方案
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BCL2-Associated Athanogene (BAG1) (AA 1-355) protein (His tag)
custom-made BAG1 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
BCL2-Associated Athanogene (BAG1) (AA 1-345) protein (His tag)custom-made BAG1 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
BCL2-Associated Athanogene (BAG1) (AA 241-345) protein (GST tag)BAG1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
BCL2-Associated Athanogene (BAG1) (AA 1-274) protein (GST tag)BAG1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
BCL2-Associated Athanogene (BAG1) (AA 1-230) proteinBAG1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % pure SDS
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应用备注
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Application Note: BAG1 Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol. BAG1 Protein is suitable for use in Western Blot. Expect a band approximately ~ 66 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.
Western Blot Dilution: User Optimized
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.2 μg/μL
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缓冲液
- BAG1 Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.
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储存条件
- -80 °C
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储存方法
- Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
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有效期
- 12 months
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- BAG1 (BCL2-Associated Athanogene (BAG1))
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别名
- BAG1
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背景
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Synonyms: BAG family molecular chaperone regulator 1 (BAG-1) (Bcl-2-associated athanogene 1), HAP, RAP46
Background: BAG1 (also known as BCL2-associated athanogene) is a membrane protein rich in glutamic acid residues that binds to BCL2 and blocks apoptosis or programmed cell death (1). The BAG1-BCL2 complex enhances the anti-apoptotic effects of BCL2 and represents a link between growth factor receptors and anti-apoptotic mechanisms (2). Overexpression of BAG1 in 3T3 fibroblasts prevents apoptosis in the presence of low serum. BAG1 has also been shown to interact with activated glucocorticoid, androgen, estrogen and progesterone receptors. Binding to these receptors by BAG1 is dependent on receptor activation. BAG1 Protein is ideal for investigators involved in Signaling Proteins, Apoptosis Proteins, AKT/PKB Pathway, Apoptosis/Autophagy, Cancer, Cardiovascular Disease, and Neurobiology research.
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NCBI登录号
- NM_004323
抗原
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