Cyclophilin-Like 4/PPIL4 (AA 1-492) (Active) protein (His tag)
This Recombinant protein is expressed in 大肠杆菌（E. Coli）.
1 image
Quick Overview for Cyclophilin-Like 4/PPIL4 (AA 1-492) (Active) protein (His tag) (ABIN667837)
抗原
Cyclophilin-Like 4/PPIL4
蛋白类型
Recombinant
生物活性
Active
宿主
人
资源
大肠杆菌（E. Coli）
应用范围
Enzyme Activity Assay (EAA), SDS-PAGE (SDS)
纯度
> 85% by SDS-PAGE
-
-
产品特性
- AA 1-492
-
标记
- His tag
-
序列
-
MAVLLETTLG DVVIDLYTEE RPRACLNFLK LCKIKYYNYC LIHNVQRDFI IQTGDPTGTG RGGESIFGQL YGDQASFFEA EKVPRIKHKK KGTVSMVNNG SDQHGSQFLI TTGENLDYLD GVHTVFGEVT EGMDIIKKIN ETFVDKDFVP YQDIRINHTV ILDDPFDDPP DLLIPDRSPE PTREQLDSGR IGADEEIDDF KGRSAEEVEE IKAEKEAKTQ AILLEMVGDL PDADIKPPEN VLFVCKLNPV TTDEDLEIIF SRFGPIRSCE VIRDWKTGES LCYAFIEFEK EEDCEKAFFK MDNVLIDDRR IHVDFSQSVA KVKWKGKGGK YTKSDFKEYE KEQDKPPNLV LKDKVKPKQD TKYDLILDEQ AEDSKSSHSH TSKKHKKKTH HCSEEKEDED YMPIKNTNQD IYREMGFGHY EEEESCWEKQ KSEKRDRTQN RSRSRSRERD GHYSNSHKSK YQTDLYERER SKKRDRSRSP KKSKDKEKSK YR -
产品特性
- PPIL4, 1-492aa, Human, His tag, E.coli
-
Biological Activity Comment
- Specific activity is > 190nmol/min/mg, and is defined as the amount of enzyme that cleaves 1umole of suc-AAPF-pNA per minute at 37C in Tris-Hcl pH8.0 using chymotrypsin
-
-
-
应用备注
- Optimal working dilution should be determined by the investigator.
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 0.5 mg/mL
-
缓冲液
- Liquid. In 20mM Tris-HCl buffer(pH 8.0) containing 10% glycerol, 2mM DTT, 0.1M NaCl.
-
储存条件
- 4 °C,-20 °C,-80 °C
-
储存方法
- Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
-
-
- Cyclophilin-Like 4/PPIL4
-
背景
- PPIL4, also known as HDCME13P, is evolutionarily conserved member of the cyclophilin-type PPIase family of proteins. ubiquitously expressed with predominant expression in kidney, PPIL4 localizes to the nucleus and contains one PPIase cyclophilin-type domain, a lysine-rich domain, a pair of bipartite nuclear targeting sequences and one RRM (RNA recognition motif) domain. The presence of the RRM domain along with nuclear targeting sequences suggests that PPIL4 may be involved in transcriptional regulation. Recombinant human PPIL4 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
-
分子量
- 59.3 kDa (512aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
-
NCBI登录号
- NP_624311
抗原
-
You are here:
