Ppa (AA 1-176) protein (His tag)
This Recombinant protein is expressed in 大肠杆菌（E. Coli）.
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Quick Overview for Ppa (AA 1-176) protein (His tag) (ABIN667500)
抗原
Ppa
蛋白类型
Recombinant
宿主
E. coli
资源
大肠杆菌（E. Coli）
应用范围
SDS-PAGE (SDS)
纯度
> 95 % by SDS - PAGE
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产品特性
- AA 1-176
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标记
- His tag
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产品特性
- ppa, 1-176aa, E.coli, His tag, E.coli
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1mg/ml (determined by Bradford assay)
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缓冲液
- Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 50mM NaCl
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储存条件
- 4 °C
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- Ppa
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背景
- Inorganic pyrophosphatase (ppa) belongs to the Ppase family. This protein is an enzyme that catalyzes the conversion of one molecule of pyrophosphate to two phosphate ions. This is a highly exergonic reaction, and therefore can be coupled to unfavorable biochemical transformations in order to drive these transformations to completion. The functionality of this enzyme plays a critical role in lipid metabolism (including lipid synthesis and degradation), calcium absorption and bone formation, and DNA synthesis, as well as other biochemical transformations. Recombinant E.coli ppa protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. Synonyms: inorganic pyrophosphatase, ECK4222, JW4185. NCBI no.: NP_418647
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分子量
- 21.9kDa (196aa), confirmed by MALDI-TOF
抗原
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