AnsA (AA 1-338) protein (His tag)
This Recombinant protein is expressed in 大肠杆菌（E. Coli）.
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Quick Overview for AnsA (AA 1-338) protein (His tag) (ABIN667462)
抗原
AnsA
蛋白类型
Recombinant
宿主
E. coli
资源
大肠杆菌（E. Coli）
应用范围
SDS-PAGE (SDS)
纯度
> 95 %
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产品特性
- AA 1-338
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标记
- His tag
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序列
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MQKKSIYVAY TGGTIGMQRS EQGYIPVSGH LQRQLALMPE FHRPEMPDFT IHEYTPLMDS SDMTPEDWQH IAEDIKAHYD DYDGFVILHG TDTMAYTASA LSFMLENLGK PVIVTGSQIP LAELRSDGQI NLLNALYVAA NYPINEVTLF FNNRLYRGNR TTKAHADGFD AFASPNLPPL LEAGIHIRRL NTPPAPHGEG ELIVHPITPQ PIGVVTIYPG ISADVVRNFL RQPVKALILR SYGVGNAPQN KAFLQELQEA SDRGIVVVNL TQCMSGKVNM GGYATGNALA HAGVIGGADM TVEATLTKLH YLLSQELDTE TIRKAMSQNL RGELTPDD -
产品特性
- ansA, 1-338aa, E.coli, His tag, E.coli
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纯化方法
- > 95% by SDS-PAGE
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- 20 mM Tris-HCl buffer ( pH 8.0) containing 10 % glycerol, 2 mM DTT
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- AnsA
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背景
- AnsA is the cytoplasmic asparaginase from Escherichia coli involved in intracellular asparagine utilization. Escherichia coli possesse two l-asparaginases: the cytoplasmic type I form (ansA) and the periplasmic type II form (ansB). AnsA (Type I asparaginase) is constitutively expressed and required for the growth of the bacteria on asparagine as the sole nitrogen source. Recombinant E. coli ansA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
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分子量
- 39.3 kDa (358aa) confirmed by MALDI-TOF
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NCBI登录号
- NP_416281
抗原
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