AnsA (AA 1-338) protein (His tag)
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- 抗原
- AnsA
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-338
- 宿主
- E. coli
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资源
- 大肠杆菌(E. Coli)
- 标记
- His tag
- 应用范围
- SDS-PAGE (SDS)
- 产品特性
- ansA, 1-338aa, E.coli, His tag, E.coli
- 纯度
- > 95 % by SDS - PAGE
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- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/ml (determined by Bradford assay)
- 缓冲液
- Liquid. In 20mM Tris-HCl buffer(pH 8.0) containing 10% glycerol, 2mM DTT.
- 储存条件
- 4 °C
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- 抗原
- AnsA
- 背景
- AnsA is the cytoplasmic asparaginase from Escherichia coli involved in intracellular asparagine utilization. Escherichia coli possesse two l-asparaginases: the cytoplasmic type I form (ansA) and the periplasmic type II form (ansB). AnsA (Type I asparaginase) is constitutively expressed and required for the growth of the bacteria on asparagine as the sole nitrogen source. Recombinant E.coli ansA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. Synonyms: Cytoplasmic L-asparaginase I, ECK1765, JW1756. NCBI no.: NP_416281
- 分子量
- 39.3 kDa (358aa), confirmed by MALDI-TOF
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