NTH (AA 1-211) protein (His tag)
This Recombinant protein is expressed in 大肠杆菌（E. Coli）.
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Quick Overview for NTH (AA 1-211) protein (His tag) (ABIN667447)
抗原
NTH
蛋白类型
Recombinant
宿主
E. coli
资源
大肠杆菌（E. Coli）
应用范围
SDS-PAGE (SDS)
纯度
> 90% by SDS-PAGE
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产品特性
- AA 1-211
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标记
- His tag
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序列
- MGSSHHHHHH SSGLVPRGSH MNKAKRLEIL TRLRENNPHP TTELNFSSPF ELLIAVLLSA QATDVSVNKA TAKLYPVANT PAAMLELGVE GVKTYIKTIG LYNSKAENII KTCRILLEQH NGEVPEDRAA LEALPGVGRK TANVVLNTAF GWPTIAVDTH IFRVCNRTQF APGKNVEQVE EKLLKVVPAE FKVDCHHWLI LHGRYTCIAR KPRCGSCIIE DLCEYKEKVD I
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产品特性
- nth, 1-211aa, E.coli, His tag, E.coli
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.25 mg/mL
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缓冲液
- Liquid. 20mM Tris-HCl buffer (pH8.0) containing 40% glycerol, 0.1M NaCl, 1mM DTT, 0.1mM PMSF
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
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- NTH
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背景
- Endonuclease III, also known as nth, is a DNA repair enzyme that has both DNA N-glycosylase activity and AP-lyase activity. The DNA N-glycosylase activity releases various damaged pyrimidines from DNA by cleaving the N-glycosidic bond, leaving an AP (apurinic/apyrimidinic) site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate. Recombinant E. coli nth protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
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分子量
- 25.7 kDa (231aa) confirmed by MALDI-TOF
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NCBI登录号
- NP_416150
抗原
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