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NTH (AA 1-211) protein (His tag)

宿主: E. coli 宿主: 大肠杆菌(E. Coli) Recombinant > 85 % by SDS - PAGE SDS
产品编号 ABIN667447
发货至: 中国
  • 抗原
    NTH
    蛋白类型
    Recombinant
    产品特性
    AA 1-211
    宿主
    E. coli
    资源
    • 1
    大肠杆菌(E. Coli)
    标记
    His tag
    应用范围
    SDS-PAGE (SDS)
    产品特性
    nth, 1-211aa, E.coli, His tag, E.coli
    纯度
    > 85 % by SDS - PAGE
  • 限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.5 mg/ml (determined by Bradford assay)
    缓冲液
    Liquid. 20mM Tris-HCl buffer (pH8.0) containing 40% glycerol, 0.1M NaCl, 1mM DTT, 0.1mM PMSF
    储存条件
    4 °C
  • 抗原
    NTH
    背景
    Nth, also known as Endonuclease III, has an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. This enzyme incises damaged DNA at cytosines, thymines and guanines. Nth acts on a damaged strand, 5' from the damaged site. This enzyme is required for the repair of both oxidative DNA damage and spontaneous mutagenic lesions. Recombinant E.coli nth protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography. Synonyms: Endonuclease III, b1633, JW1625, DNA-(apurinic or apyrimidinic site) lyase. NCBI no.: NP_416150
    分子量
    25.7 kDa (231aa) confirmed by MALDI-TOF
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