NanA (AA 1-297) protein (His tag)
This Recombinant protein is expressed in 大肠杆菌（E. Coli）.
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Quick Overview for NanA (AA 1-297) protein (His tag) (ABIN667434)
抗原
NanA
蛋白类型
Recombinant
宿主
E. coli
资源
大肠杆菌（E. Coli）
应用范围
SDS-PAGE (SDS)
纯度
> 95 % by SDS - PAGE
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产品特性
- AA 1-297
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标记
- His tag
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产品特性
- nanA, 1-297aa, E.coli, His tag, E.coli
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/ml (determined by Bradford assay)
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缓冲液
- Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol
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储存条件
- 4 °C
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- NanA
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背景
- NanA, also known as N-acetylneuraminate lyase, belongs to the family of lyases, specifically the oxo-acid-lyases, which cleave carbon-carbon bonds. NanA catalyzes the cleavage of N-acetylneuraminic acid (sialic acid) to form pyruvate and N-acetyl-D-mannosamine. This protein was inhibited by reduction with NaBH4 in the presence of the substrate, indicating that it belongs to the Schiff-base-forming Class I aldolases. NanA was strongly inhibited by Cu2+ ions, p-chloromercuribenzoate and N-bromosuccinimide, and also inhibited competitively by the reaction product, pyruvate, and its structurally related compounds, dihydroxyacetone and DL-glyceraldehyde. Recombinant E.coli nanA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography. Synonyms: N-acetylneuraminate lyase, npl. NCBI no.: NP_417692
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分子量
- 34.7 kDa (317aa) confirmed by MALDI-TOF
抗原
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