NanA (AA 1-297) protein (His tag)
宿主: E. coli
宿主: 大肠杆菌（E. Coli）
Recombinant
> 95 % by SDS - PAGE
SDS
1 image
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- 抗原
- NanA
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-297
- 宿主
- E. coli
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资源
- 大肠杆菌（E. Coli）
- 标记
- His tag
- 应用范围
- SDS-PAGE (SDS)
- 产品特性
- nanA, 1-297aa, E.coli, His tag, E.coli
- 纯度
- > 95 % by SDS - PAGE
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- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/ml (determined by Bradford assay)
- 缓冲液
- Liquid. 20mM Tris-HCl buffer (pH8.0) containing 20% glycerol
- 储存条件
- 4 °C
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- 抗原
- NanA
- 背景
- NanA, also known as N-acetylneuraminate lyase, belongs to the family of lyases, specifically the oxo-acid-lyases, which cleave carbon-carbon bonds. NanA catalyzes the cleavage of N-acetylneuraminic acid (sialic acid) to form pyruvate and N-acetyl-D-mannosamine. This protein was inhibited by reduction with NaBH4 in the presence of the substrate, indicating that it belongs to the Schiff-base-forming Class I aldolases. NanA was strongly inhibited by Cu2+ ions, p-chloromercuribenzoate and N-bromosuccinimide, and also inhibited competitively by the reaction product, pyruvate, and its structurally related compounds, dihydroxyacetone and DL-glyceraldehyde. Recombinant E.coli nanA protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography. Synonyms: N-acetylneuraminate lyase, npl. NCBI no.: NP_417692
- 分子量
- 34.7 kDa (317aa) confirmed by MALDI-TOF
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