HchA (AA 1-283) protein (His tag)
This Recombinant protein is expressed in 大肠杆菌（E. Coli）.
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Quick Overview for HchA (AA 1-283) protein (His tag) (ABIN667426)
抗原
HchA
蛋白类型
Recombinant
宿主
E. coli
资源
大肠杆菌（E. Coli）
应用范围
SDS-PAGE (SDS)
纯度
> 95 %
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产品特性
- AA 1-283
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标记
- His tag
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序列
- MGSSHHHHHH SSGLVPRGSH MTVQTSKNPQ VDIAEDNAFF PSEYSLSQYT SPVSDLDGVD YPKPYRGKHK ILVIAADERY LPTDNGKLFS TGNHPIETLL PLYHLHAAGF EFEVATISGL MTKFEYWAMP HKDEKVMPFF EQHKSLFRNP KKLADVVASL NADSEYAAIF VPGGHGALIG LPESQDVAAA LQWAIKNDRF VISLCHGPAA FLALRHGDNP LNGYSICAFP DAADKQTPEI GYMPGHLTWY FGEELKKMGM NIINDDITGR VHKDRKLLTG DSPFAANALG KLAAQEMLAA YAG
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产品特性
- hchA, 1-283aa, E.coli, His tag, E.coli
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纯化方法
- > 95% by SDS-PAGE
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- 20 mM Tris-HCl buffer ( pH 8.0) containing 20 % glycerol, 0.1M NaCl,1 mM DTT
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- HchA
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背景
- HchA, also known as, Escherichia coli Hsp31, is a homodimeric member of the ThiI/DJ-1/PfpI superfamily that combines molecular chaperone and aminopeptidase activities. It uses temperature-induced exposure of structured hydrophobic domains to capture and stabilize early unfolding protein intermediates under severe thermal stress. Recombinant E. coli hchA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
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分子量
- 33.3 kDa (303aa) confirmed by MALDI-TOF
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NCBI登录号
- NP_416476
抗原
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