LIF
宿主: 人
宿主: HEK-293 Cells
Recombinant
The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
应用备注
Optimal working dilution should be determined by the investigator.
限制
仅限研究用
状态
Liquid
浓度
1 mg/mL
缓冲液
20 mM Tris-HCl buffer ( pH 8.0) containing 1 mM DTT, 20 % glycerol, 50 mM NaCl
储存条件
4 °C,-20 °C,-80 °C
储存方法
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
抗原
LIF
(Leukemia Inhibitory Factor (LIF))
别名
LIF
背景
Leukemia inhibitory factor, also known as LIF, is a pleiotropic cytokine that is expressed by a wide variety of cells including activated T lymphocytes, monocytes, mast cells and neuronal cells. It is involved in the induction of hematopoietic differentiation in normal and myeloid leukemia cells, induction of neuronal cell differentiation, regulator of mesenchymal to epithelial conversion during kidney development, and may also have a role in immune tolerance at the maternal-fetal interface. Recombinant human LIF protein, fused to His GST-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.