NFNB (AA 1-217) protein (His tag)
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- 抗原
- NFNB
- 蛋白类型
- Recombinant
- 产品特性
- AA 1-217
- 宿主
- E. coli
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资源
- 大肠杆菌(E. Coli)
- 标记
- His tag
- 应用范围
- SDS-PAGE (SDS)
- 产品特性
- NFNB, 1-217aa, E.coli, His tag, E.coli
- 纯度
- > 95 % by SDS - PAGE
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- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1 mg/ml (determined by Bradford assay)
- 缓冲液
- Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 50mM Nacl, 1mM DTT, 10% glycerol
- 储存条件
- 4 °C
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- 抗原
- NFNB
- 背景
- NFNB, also known as NFSB, shows the ability to reduce quinines. This protein is an enzyme for activating prodrugs in antibody directed enzyme prodrug therapy. It also capable of reducing nitrofurazone, quinones and the anti-tumor agent CB1954 (5-(aziridin-1-yl)-2,4-dinitrobenzamide). The reduction of CB1954 results in the generation of cytotoxic species. Recombinant E.coli NFNB protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques. Synonyms: dprA, nfsB, nfsI, ntr, dihydropteridine reductase, NAD(P)H-dependent, oxygen-insensitive. NCBI no.: NP_415110
- 分子量
- 26.0 kDa (237aa) confirmed by MALDI-TOF
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