SAE1 Protein (AA 1-346) (His-T7)
Quick Overview for SAE1 Protein (AA 1-346) (His-T7) (ABIN667139)
抗原
See all SAE1 蛋白蛋白类型
宿主
资源
应用范围
纯度
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产品特性
- AA 1-346
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标记
- This SAE1 protein is labelled with His-T7.
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序列
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MVEKEEAG GGISEEEAAQ YDRQIRLWGL EAQKRLRASR VLLVGLKGLG AEIAKNLILA GVKGLTMLDH EQVTPEDPGA QFLIRTGSVG RNRAEASLER AQNLNPMVDV KVDTEDIEKK PESFFTQFDA VCLTCCSRDV IVKVDQICHK NSIKFFTGDV FGYHGYTFAN LGEHEFVEEK TKVAKVSQGV EDGPDTKRAK LDSSETTMVK KKVVFCPVKE ALEVDWSSEK AKAALKRTTS DYFLLQVLLK FRTDKGRDPS SDTYEEDSEL LLQIRNDVLD SLGISPDLLP EDFVRYCFSE MAPVCAVVGG ILAQEIVKAL SQRDPPHNNF FFFDGMKGNG IVECLGPK -
产品特性
- SAE1, 1-346aa, Human, His-T7 tag, E.coli
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!探索我们的预定义定制蛋白和定制蛋白服务！Product表达系统ConjugateOrigin价格从表达系统 HEK-293 CellsConjugate His tagOrigin Human价格从 106,314.67 ¥表达系统 Cell-free protein synthesis (CFPS)Conjugate Strep TagOrigin Human价格从 172,528.32 ¥您的项目需要进一步定制吗？联系我们，了解我们的定制蛋白解决方案
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SUMO1 Activating Enzyme Subunit 1 (SAE1) (AA 1-350) protein (His tag)
custom-made SAE1 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
SUMO1 Activating Enzyme Subunit 1 (SAE1) (AA 1-346) protein (His tag)custom-made SAE1 宿主: 人 宿主: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
SUMO1 Activating Enzyme Subunit 1 (SAE1) (AA 1-346) protein (His tag)SAE1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
SUMO1 Activating Enzyme Subunit 1 (SAE1) (AA 1-346) protein (GST tag)SAE1 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AP, AA
SUMO1 Activating Enzyme Subunit 1 (SAE1) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)SAE1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
SUMO1 Activating Enzyme Subunit 1 (SAE1) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)SAE1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- Liquid. In 20 mM Tris-HCl buffer (pH8.0) containing 1mM DTT, 10% glycerol
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
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- SAE1 (SUMO1 Activating Enzyme Subunit 1 (SAE1))
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别名
- SUMO E1/SAE1
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背景
- SAE1, also known as AOS1, belongs to the ubiquitin-activating E1 family of proteins and plays an important role in the first step of the uBL1 conjugation pathway. Proteins conjugated to ub are marked for progressive degradation by the 26S Proteasome. SAE1, which is a dimeric enzyme, functions as a uBLI E1 ligase mediating the ATP-dependent activation of uBL1. This protein can bind with uBLE1A and uBLE1B to form a heterodimer which can bind uBL1. Recombinant human SAE1 protein, fused to His-T7-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
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分子量
- 42.2 kDa (378aa) confirmed by MALDI-TOF
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NCBI登录号
- NP_005491
抗原
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