Quick Overview for SOD1 Protein (AA 1-154) (ABIN667079)
抗原
See all SOD1 蛋白
SOD1
(Superoxide Dismutase 1, Soluble (SOD1))
蛋白类型
Recombinant
生物活性
Active
宿主
人
资源
大肠杆菌（E. Coli）
应用范围
SDS-PAGE (SDS), Enzyme Activity Assay (EAA)
纯度
> 95 %
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产品特性
- AA 1-154
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序列
- MATKAVCVLK GDGPVQGIIN FEQKESNGPV KVWGSIKGLT EGLHGFHVHE FGDNTAGCTS AGPHFNPLSR KHGGPKDEER HVGDLGNVTA DKDGVADVSI EDSVISLSGD HCIIGRTLVV HEKADDLGKG GNEESTKTGN AGSRLACGVI GIAQ
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产品特性
- SOD1, 1-154aa, Human, Recombinant, E.coli
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纯化方法
- > 95% by SDS-PAGE
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内毒素水平
- < 1 EU per 1ug of protein (determined by LAL method)
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Biological Activity Comment
- Specific activity is > 500unit/mg, in which one unit will inhibit the rate of reduction of cytochrome c by 50% in a coupled system, using xanthine and Xanthine oxidase at pH 7.5 at 25C.
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Superoxide Dismutase 1, Soluble (SOD1) (AA 1-154) protein (GST tag)
SOD1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Superoxide Dismutase 1, Soluble (SOD1) (AA 1-154) (Active) proteinSOD1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % pure SDS Active
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- 20 mM Tris-HCl buffer ( pH 7.5) containing 10 % glycerol
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- SOD1 (Superoxide Dismutase 1, Soluble (SOD1))
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别名
- SOD1/Cu-Zn SOD
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背景
- Superoxide dismutase 1 (SOD1) binds copper and zinc ions and is one of three isozymes responsible for destroying free superoxide radicals in the body. The encoded protein neutralizes supercharged oxygen molecules, which can damage cells if their levels are not controlled. Mutations in SOD1 cause a form of familial amyotrophic lateral sclerosis (ALS). Recombinant SOD1 was expressed in E. coli and purified by conventional chromatography techniques.
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分子量
- 15.9 kDa (154aa) confirmed by MALDI-TOF
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NCBI登录号
- NP_000445
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途径
- Sensory Perception of Sound, Transition Metal Ion Homeostasis
抗原
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