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SlyD (AA 1-196) (Active) 蛋白

This Recombinant not_set protein is expressed in 大肠杆菌(E. Coli).
产品编号 ABIN667072
发货至: 中国
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Quick Overview for SlyD (AA 1-196) (Active) 蛋白 (ABIN667072)

抗原

SlyD

蛋白类型

Recombinant

生物活性

Active

宿主

E. coli

资源

  • 1
大肠杆菌(E. Coli)

应用范围

SDS-PAGE (SDS), Enzyme Activity Assay (EAA)

纯度

> 95 %
  • 产品特性

    AA 1-196

    序列

    MKVAKDLVVS LAYQVRTEDG VLVDESPVSA PLDYLHGHGS LISGLETALE GHEVGDKFDV AVGANDAYGQ YDENLVQRVP KDVFMGVDEL QVGMRFLAET DQGPVPVEIT AVEDDHVVVD GNHMLAGQNL KFNVEVVAIR EATEEELAHG HVHGAHDHHH DHDHDGCCGG HGHDHGHEHG GEGCCGGKGN GGCGCH

    产品特性

    SlyD,1-196aa, E.coli, Recombinant, E.coli

    纯化方法

    > 95% by SDS-PAGE

    Biological Activity Comment

    Specific activity is > 700nmol/min/mg, and is defined as the amount of enzyme cleaves 1nmol of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.
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  • 应用备注

    Optimal working dilution should be determined by the investigator.

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    1 mg/mL

    缓冲液

    20 mM Tris-HCl buffer ( pH 7.5)

    储存条件

    4 °C,-20 °C,-80 °C

    储存方法

    Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
  • 抗原

    SlyD

    背景

    SlyD is a putative folding helper protein from the Escherichia coli cytosol, which consists of an N-terminal prolyl isomerase domain of the FKBP type and a presumably unstructured C-terminal tail. It is involved in the biosynthesis of the metal cluster in the [NiFe]-hydrogenase enzymes, and exhibits several activities including that of a peptidyl-prolyl isomerase. Recombinant E. Coli slyD was expressed in E. coli and purified by conventional chromatography.

    分子量

    21 kDa (196aa) confirmed by MALDI-TOF

    NCBI登录号

    NP_755987
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