SlyD (AA 1-196) (Active) 蛋白
Our Local Distributor
北京 101111
Quick Overview for SlyD (AA 1-196) (Active) 蛋白 (ABIN667072)
抗原
蛋白类型
生物活性
宿主
资源
应用范围
纯度
-
-
产品特性
- AA 1-196
-
序列
- MKVAKDLVVS LAYQVRTEDG VLVDESPVSA PLDYLHGHGS LISGLETALE GHEVGDKFDV AVGANDAYGQ YDENLVQRVP KDVFMGVDEL QVGMRFLAET DQGPVPVEIT AVEDDHVVVD GNHMLAGQNL KFNVEVVAIR EATEEELAHG HVHGAHDHHH DHDHDGCCGG HGHDHGHEHG GEGCCGGKGN GGCGCH
-
产品特性
- SlyD,1-196aa, E.coli, Recombinant, E.coli
-
纯化方法
- > 95% by SDS-PAGE
-
Biological Activity Comment
- Specific activity is > 700nmol/min/mg, and is defined as the amount of enzyme cleaves 1nmol of suc-AAPF-pNA per minute at 37C in Tris-HCl pH 8.0 using chymotrypsin.
-
-
-
-
应用备注
- Optimal working dilution should be determined by the investigator.
-
限制
- 仅限研究用
-
-
-
状态
- Liquid
-
浓度
- 1 mg/mL
-
缓冲液
- 20 mM Tris-HCl buffer ( pH 7.5)
-
储存条件
- 4 °C,-20 °C,-80 °C
-
储存方法
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
-
-
- SlyD
-
背景
- SlyD is a putative folding helper protein from the Escherichia coli cytosol, which consists of an N-terminal prolyl isomerase domain of the FKBP type and a presumably unstructured C-terminal tail. It is involved in the biosynthesis of the metal cluster in the [NiFe]-hydrogenase enzymes, and exhibits several activities including that of a peptidyl-prolyl isomerase. Recombinant E. Coli slyD was expressed in E. coli and purified by conventional chromatography.
-
分子量
- 21 kDa (196aa) confirmed by MALDI-TOF
-
NCBI登录号
- NP_755987
抗原
-