CERT1 (AA 347-598) protein (His tag)
This Recombinant CERT1 protein is expressed in 大肠杆菌（E. Coli）.
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产品编号 ABIN667012
发货至:
中国
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Contact Info
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中国
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北京 101111
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Quick Overview for CERT1 (AA 347-598) protein (His tag) (ABIN667012)
抗原
CERT1
蛋白类型
Recombinant
宿主
人
资源
大肠杆菌（E. Coli）
应用范围
SDS-PAGE (SDS)
纯度
> 95 %
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产品特性
- AA 347-598
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标记
- His tag
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序列
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LH WPTSLPSGDA FSSVGTHRFV QKVEEMVQNH MTYSLQDVGG DANWQLVVEE GEMKVYRREV EENGIVLDPL KATHAVKGVT GHEVCNYFWN VDVRNDWETT IENFHVVETL ADNAIIIYQT HKRVWPASQR DVLYLSVIRK IPALTENDPE TWIVCNFSVD HDSAPLNNRC VRAKINVAMI CQTLVSPPEG NQEISRDNIL CKITYVANVN PGGWAPASVL RAVAKREYPK FLKRFTSYVQ EKTAGKPILF -
产品特性
- CERT, 347-598aa, Human, His tag, E.coli
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纯化方法
- > 95% by SDS-PAGE
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CERT1 (AA 347-598) protein (His-IF2DI Tag)
宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 90 % as determined by SDS-PAGE. ELISA, WB
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应用备注
- Optimal working dilution should be determined by the investigator.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- 20 mM Tris-HCl buffer ( pH 8.0) containing 10 % glycerol 0.1M NaCl
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储存条件
- 4 °C,-20 °C,-80 °C
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储存方法
- Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
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- CERT1
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背景
- CERT is a kinase that specifically phosphorylates the N-terminal region of the non-collagenous domain of the alpha 3 chain of type IV collagen, known as the Goodpasture antigen. Goodpasture disease is the result of an autoimmune response directed at this antigen. One isoform of this protein is also involved in ceramide intracellular transport. Recombinant human CERT protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
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分子量
- 32.9 kDa (290aa) confirmed by MALDI-TOF
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NCBI登录号
- NP_112729
抗原
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